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Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effect...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480961/ https://www.ncbi.nlm.nih.gov/pubmed/35977050 http://dx.doi.org/10.1002/brb3.2715 |
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author | Zou, Jiao Shang, Weilong Yang, Ling Liu, Tianyao Wang, Lian Li, Xin Zhao, Jinghui Rao, Xiancai Gao, Junwei Fan, Xiaotang |
author_facet | Zou, Jiao Shang, Weilong Yang, Ling Liu, Tianyao Wang, Lian Li, Xin Zhao, Jinghui Rao, Xiancai Gao, Junwei Fan, Xiaotang |
author_sort | Zou, Jiao |
collection | PubMed |
description | Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effects of S. aureus infection on abnormal behaviors, we conducted the present study. Methods: A S. aureus USA300‐infected mouse model was established using bacterial suspension injection into tail vein. A series of behavioral tests were performed after USA300 infection. The expression of cytokines was detected in serum and mPFC. The number and some morphological parameters of microglia were also evaluated by immunofluorescence staining. Results: Anxiety‐like behaviors, instead of locomotor activity impairment or depression‐like behaviors, were observed in mice infected with S. aureus USA300 compared with control. S. aureus USA300 infection caused overexpression of IL‐6, TNF‐α, and IL‐1β in serum, resulted in microglial over‐activation and excessive release of proinflammatory cytokines in the mPFC. In addition, overexpression of TLR2 accompanied by increased GLS1 and p‐STAT3 was observed in the mPFC of mice infected with S. aureus USA300. Conclusion: This study provides evidence that S. aureus USA300 infection can lead to neuroinflammation in the mPFC of mice, which may contribute to the development of anxiety. |
format | Online Article Text |
id | pubmed-9480961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94809612022-09-28 Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 Zou, Jiao Shang, Weilong Yang, Ling Liu, Tianyao Wang, Lian Li, Xin Zhao, Jinghui Rao, Xiancai Gao, Junwei Fan, Xiaotang Brain Behav Original Articles Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effects of S. aureus infection on abnormal behaviors, we conducted the present study. Methods: A S. aureus USA300‐infected mouse model was established using bacterial suspension injection into tail vein. A series of behavioral tests were performed after USA300 infection. The expression of cytokines was detected in serum and mPFC. The number and some morphological parameters of microglia were also evaluated by immunofluorescence staining. Results: Anxiety‐like behaviors, instead of locomotor activity impairment or depression‐like behaviors, were observed in mice infected with S. aureus USA300 compared with control. S. aureus USA300 infection caused overexpression of IL‐6, TNF‐α, and IL‐1β in serum, resulted in microglial over‐activation and excessive release of proinflammatory cytokines in the mPFC. In addition, overexpression of TLR2 accompanied by increased GLS1 and p‐STAT3 was observed in the mPFC of mice infected with S. aureus USA300. Conclusion: This study provides evidence that S. aureus USA300 infection can lead to neuroinflammation in the mPFC of mice, which may contribute to the development of anxiety. John Wiley and Sons Inc. 2022-08-17 /pmc/articles/PMC9480961/ /pubmed/35977050 http://dx.doi.org/10.1002/brb3.2715 Text en © 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zou, Jiao Shang, Weilong Yang, Ling Liu, Tianyao Wang, Lian Li, Xin Zhao, Jinghui Rao, Xiancai Gao, Junwei Fan, Xiaotang Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title | Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title_full | Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title_fullStr | Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title_full_unstemmed | Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title_short | Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 |
title_sort | microglia activation in the mpfc mediates anxiety‐like behaviors caused by staphylococcus aureus strain usa300 |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480961/ https://www.ncbi.nlm.nih.gov/pubmed/35977050 http://dx.doi.org/10.1002/brb3.2715 |
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