Cargando…

Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300

Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effect...

Descripción completa

Detalles Bibliográficos
Autores principales: Zou, Jiao, Shang, Weilong, Yang, Ling, Liu, Tianyao, Wang, Lian, Li, Xin, Zhao, Jinghui, Rao, Xiancai, Gao, Junwei, Fan, Xiaotang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480961/
https://www.ncbi.nlm.nih.gov/pubmed/35977050
http://dx.doi.org/10.1002/brb3.2715
_version_ 1784791157760327680
author Zou, Jiao
Shang, Weilong
Yang, Ling
Liu, Tianyao
Wang, Lian
Li, Xin
Zhao, Jinghui
Rao, Xiancai
Gao, Junwei
Fan, Xiaotang
author_facet Zou, Jiao
Shang, Weilong
Yang, Ling
Liu, Tianyao
Wang, Lian
Li, Xin
Zhao, Jinghui
Rao, Xiancai
Gao, Junwei
Fan, Xiaotang
author_sort Zou, Jiao
collection PubMed
description Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effects of S. aureus infection on abnormal behaviors, we conducted the present study. Methods: A S. aureus USA300‐infected mouse model was established using bacterial suspension injection into tail vein. A series of behavioral tests were performed after USA300 infection. The expression of cytokines was detected in serum and mPFC. The number and some morphological parameters of microglia were also evaluated by immunofluorescence staining. Results: Anxiety‐like behaviors, instead of locomotor activity impairment or depression‐like behaviors, were observed in mice infected with S. aureus USA300 compared with control. S. aureus USA300 infection caused overexpression of IL‐6, TNF‐α, and IL‐1β in serum, resulted in microglial over‐activation and excessive release of proinflammatory cytokines in the mPFC. In addition, overexpression of TLR2 accompanied by increased GLS1 and p‐STAT3 was observed in the mPFC of mice infected with S. aureus USA300. Conclusion: This study provides evidence that S. aureus USA300 infection can lead to neuroinflammation in the mPFC of mice, which may contribute to the development of anxiety.
format Online
Article
Text
id pubmed-9480961
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-94809612022-09-28 Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300 Zou, Jiao Shang, Weilong Yang, Ling Liu, Tianyao Wang, Lian Li, Xin Zhao, Jinghui Rao, Xiancai Gao, Junwei Fan, Xiaotang Brain Behav Original Articles Introduction: Staphylococcus aureus (S. aureus) is considered as one of the major causative agents of serious hospital‐ and community‐acquired infections. Recent studies have reported that S. aureus infection induced neuroinflammation and was linked with some mental disorders. To evaluate the effects of S. aureus infection on abnormal behaviors, we conducted the present study. Methods: A S. aureus USA300‐infected mouse model was established using bacterial suspension injection into tail vein. A series of behavioral tests were performed after USA300 infection. The expression of cytokines was detected in serum and mPFC. The number and some morphological parameters of microglia were also evaluated by immunofluorescence staining. Results: Anxiety‐like behaviors, instead of locomotor activity impairment or depression‐like behaviors, were observed in mice infected with S. aureus USA300 compared with control. S. aureus USA300 infection caused overexpression of IL‐6, TNF‐α, and IL‐1β in serum, resulted in microglial over‐activation and excessive release of proinflammatory cytokines in the mPFC. In addition, overexpression of TLR2 accompanied by increased GLS1 and p‐STAT3 was observed in the mPFC of mice infected with S. aureus USA300. Conclusion: This study provides evidence that S. aureus USA300 infection can lead to neuroinflammation in the mPFC of mice, which may contribute to the development of anxiety. John Wiley and Sons Inc. 2022-08-17 /pmc/articles/PMC9480961/ /pubmed/35977050 http://dx.doi.org/10.1002/brb3.2715 Text en © 2022 The Authors. Brain and Behavior published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zou, Jiao
Shang, Weilong
Yang, Ling
Liu, Tianyao
Wang, Lian
Li, Xin
Zhao, Jinghui
Rao, Xiancai
Gao, Junwei
Fan, Xiaotang
Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title_full Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title_fullStr Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title_full_unstemmed Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title_short Microglia activation in the mPFC mediates anxiety‐like behaviors caused by Staphylococcus aureus strain USA300
title_sort microglia activation in the mpfc mediates anxiety‐like behaviors caused by staphylococcus aureus strain usa300
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9480961/
https://www.ncbi.nlm.nih.gov/pubmed/35977050
http://dx.doi.org/10.1002/brb3.2715
work_keys_str_mv AT zoujiao microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT shangweilong microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT yangling microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT liutianyao microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT wanglian microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT lixin microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT zhaojinghui microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT raoxiancai microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT gaojunwei microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300
AT fanxiaotang microgliaactivationinthempfcmediatesanxietylikebehaviorscausedbystaphylococcusaureusstrainusa300