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High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity

Engineered nanomaterials pose occupational health and environmental concerns as they possess unique physical and chemical properties that can contribute to toxicity. High throughput toxicity screening methods are needed to address the increasing number of nanomaterials in production. Here we used a...

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Detalles Bibliográficos
Autores principales: Carbaugh, Chance M., van der Schalie, William H., Widder, Mark W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481008/
https://www.ncbi.nlm.nih.gov/pubmed/36112591
http://dx.doi.org/10.1371/journal.pone.0274011
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author Carbaugh, Chance M.
van der Schalie, William H.
Widder, Mark W.
author_facet Carbaugh, Chance M.
van der Schalie, William H.
Widder, Mark W.
author_sort Carbaugh, Chance M.
collection PubMed
description Engineered nanomaterials pose occupational health and environmental concerns as they possess unique physical and chemical properties that can contribute to toxicity. High throughput toxicity screening methods are needed to address the increasing number of nanomaterials in production. Here we used a zebrafish photomotor response (PMR) test to evaluate a set of fifteen nanomaterials with military relevance. Automated dechorionation of zebrafish embryos was used to enhance nanomaterials bioavailability. Optimal PMR activity in zebrafish embryos was found at 30–31 hours post-fertilization (hpf). Behavioral and toxicological responses were measured at 30 and 120 hpf; behavioral responses were found for thirteen of the fifteen nanomaterials and acute toxicity (LC50) levels for nine of the fifteen nanomaterials below the maximum test concentration of 500 μg/ml. Physico-chemical characterization of the nanomaterials detected endotoxin and bacterial contamination in two of the tested samples, which may have contributed to observed toxicity and reinforces the need for physical and chemical characterization of nanomaterials use in toxicity testing. The zebrafish PMR test, together with automated dechorionation, provides an initial rapid assessment of the behavioral effects and toxicity of engineered nanomaterials that can be followed up by physico-chemical characterization if toxicity is detected, reducing the amount of time and monetary constraints of physico-chemical testing.
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spelling pubmed-94810082022-09-17 High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity Carbaugh, Chance M. van der Schalie, William H. Widder, Mark W. PLoS One Research Article Engineered nanomaterials pose occupational health and environmental concerns as they possess unique physical and chemical properties that can contribute to toxicity. High throughput toxicity screening methods are needed to address the increasing number of nanomaterials in production. Here we used a zebrafish photomotor response (PMR) test to evaluate a set of fifteen nanomaterials with military relevance. Automated dechorionation of zebrafish embryos was used to enhance nanomaterials bioavailability. Optimal PMR activity in zebrafish embryos was found at 30–31 hours post-fertilization (hpf). Behavioral and toxicological responses were measured at 30 and 120 hpf; behavioral responses were found for thirteen of the fifteen nanomaterials and acute toxicity (LC50) levels for nine of the fifteen nanomaterials below the maximum test concentration of 500 μg/ml. Physico-chemical characterization of the nanomaterials detected endotoxin and bacterial contamination in two of the tested samples, which may have contributed to observed toxicity and reinforces the need for physical and chemical characterization of nanomaterials use in toxicity testing. The zebrafish PMR test, together with automated dechorionation, provides an initial rapid assessment of the behavioral effects and toxicity of engineered nanomaterials that can be followed up by physico-chemical characterization if toxicity is detected, reducing the amount of time and monetary constraints of physico-chemical testing. Public Library of Science 2022-09-16 /pmc/articles/PMC9481008/ /pubmed/36112591 http://dx.doi.org/10.1371/journal.pone.0274011 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Carbaugh, Chance M.
van der Schalie, William H.
Widder, Mark W.
High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title_full High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title_fullStr High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title_full_unstemmed High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title_short High throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
title_sort high throughput embryonic zebrafish test with automated dechorionation to evaluate nanomaterial toxicity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481008/
https://www.ncbi.nlm.nih.gov/pubmed/36112591
http://dx.doi.org/10.1371/journal.pone.0274011
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