Single- and duplex TaqMan-quantitative PCR for determining the copy numbers of integrated selection markers during site-specific mutagenesis in Toxoplasma gondii by CRISPR-Cas9

Herein, we developed a single and a duplex TaqMan quantitative PCR (qPCR) for absolute quantification of copy numbers of integrated dihydrofolate reductase-thymidylate synthase (mdhfr-ts) drug selectable marker for pyrimethamine resistance in Toxoplasma gondii knockouts (KOs). The single TaqMan qPCR...

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Detalles Bibliográficos
Autores principales: Hänggeli, Kai Pascal Alexander, Hemphill, Andrew, Müller, Norbert, Schimanski, Bernd, Olias, Philipp, Müller, Joachim, Boubaker, Ghalia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481009/
https://www.ncbi.nlm.nih.gov/pubmed/36112587
http://dx.doi.org/10.1371/journal.pone.0271011

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