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4D reconstruction of murine developmental trajectories using spherical harmonics

Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to r...

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Detalles Bibliográficos
Autores principales: Dalmasso, Giovanni, Musy, Marco, Niksic, Martina, Robert-Moreno, Alexandre, Badía-Careaga, Claudio, Sanz-Ezquerro, Juan Jose, Sharpe, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481268/
https://www.ncbi.nlm.nih.gov/pubmed/36055247
http://dx.doi.org/10.1016/j.devcel.2022.08.005
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author Dalmasso, Giovanni
Musy, Marco
Niksic, Martina
Robert-Moreno, Alexandre
Badía-Careaga, Claudio
Sanz-Ezquerro, Juan Jose
Sharpe, James
author_facet Dalmasso, Giovanni
Musy, Marco
Niksic, Martina
Robert-Moreno, Alexandre
Badía-Careaga, Claudio
Sanz-Ezquerro, Juan Jose
Sharpe, James
author_sort Dalmasso, Giovanni
collection PubMed
description Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to recreate its continuous evolution in time and space from a set of 3D volumetric images. Our method is based on the remapping of shape data into the space of the coefficients of a spherical harmonics expansion where a smooth interpolation over time is simpler. We tested our approach on mouse limb buds and embryonic hearts. A key advantage of this method is that the resulting 4D trajectory can take advantage of all the available data while also being able to interpolate well through time intervals for which there are little or no data. This allows for a quantitative, data-driven 4D description of mouse limb morphogenesis.
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spelling pubmed-94812682022-09-21 4D reconstruction of murine developmental trajectories using spherical harmonics Dalmasso, Giovanni Musy, Marco Niksic, Martina Robert-Moreno, Alexandre Badía-Careaga, Claudio Sanz-Ezquerro, Juan Jose Sharpe, James Dev Cell Technology Normal organogenesis cannot be recapitulated in vitro for mammalian organs, unlike in species including Drosophila and zebrafish. Available 3D data in the form of ex vivo images only provide discrete snapshots of the development of an organ morphology. Here, we propose a computer-based approach to recreate its continuous evolution in time and space from a set of 3D volumetric images. Our method is based on the remapping of shape data into the space of the coefficients of a spherical harmonics expansion where a smooth interpolation over time is simpler. We tested our approach on mouse limb buds and embryonic hearts. A key advantage of this method is that the resulting 4D trajectory can take advantage of all the available data while also being able to interpolate well through time intervals for which there are little or no data. This allows for a quantitative, data-driven 4D description of mouse limb morphogenesis. Cell Press 2022-09-12 /pmc/articles/PMC9481268/ /pubmed/36055247 http://dx.doi.org/10.1016/j.devcel.2022.08.005 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technology
Dalmasso, Giovanni
Musy, Marco
Niksic, Martina
Robert-Moreno, Alexandre
Badía-Careaga, Claudio
Sanz-Ezquerro, Juan Jose
Sharpe, James
4D reconstruction of murine developmental trajectories using spherical harmonics
title 4D reconstruction of murine developmental trajectories using spherical harmonics
title_full 4D reconstruction of murine developmental trajectories using spherical harmonics
title_fullStr 4D reconstruction of murine developmental trajectories using spherical harmonics
title_full_unstemmed 4D reconstruction of murine developmental trajectories using spherical harmonics
title_short 4D reconstruction of murine developmental trajectories using spherical harmonics
title_sort 4d reconstruction of murine developmental trajectories using spherical harmonics
topic Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481268/
https://www.ncbi.nlm.nih.gov/pubmed/36055247
http://dx.doi.org/10.1016/j.devcel.2022.08.005
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