gDesigner: computational design of synthetic gRNAs for Cas12a-based transcriptional repression in mammalian cells

Synthetic networks require complex intertwined genetic regulation often relying on transcriptional activation or repression of target genes. CRISPRi-based transcription factors facilitate the programmable modulation of endogenous or synthetic promoter activity and the process can be optimised by usi...

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Detalles Bibliográficos
Autores principales: Crone, Michael A., MacDonald, James T., Freemont, Paul S., Siciliano, Velia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481559/
https://www.ncbi.nlm.nih.gov/pubmed/36114193
http://dx.doi.org/10.1038/s41540-022-00241-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481559/
https://www.ncbi.nlm.nih.gov/pubmed/36114193
http://dx.doi.org/10.1038/s41540-022-00241-w

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