Cargando…

α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor

BACKGROUND: This study investigated the effect of central administration of α-pinene and the interaction of α-pinene with GABAA receptor on pulpal nociception-induced changes in learning and memory performances in rats. MATERIALS AND METHODS: Sixty-six adult male Wistar rats were used. Pulpal nocice...

Descripción completa

Detalles Bibliográficos
Autores principales: Rafie, Forouzan, Kooshki, Razieh, Abbasnejad, Mehdi, Rahbar, Iran, Raoof, Maryam, Nekouei, Amir Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482378/
https://www.ncbi.nlm.nih.gov/pubmed/36124022
http://dx.doi.org/10.4103/abr.abr_139_21
_version_ 1784791441320443904
author Rafie, Forouzan
Kooshki, Razieh
Abbasnejad, Mehdi
Rahbar, Iran
Raoof, Maryam
Nekouei, Amir Hossein
author_facet Rafie, Forouzan
Kooshki, Razieh
Abbasnejad, Mehdi
Rahbar, Iran
Raoof, Maryam
Nekouei, Amir Hossein
author_sort Rafie, Forouzan
collection PubMed
description BACKGROUND: This study investigated the effect of central administration of α-pinene and the interaction of α-pinene with GABAA receptor on pulpal nociception-induced changes in learning and memory performances in rats. MATERIALS AND METHODS: Sixty-six adult male Wistar rats were used. Pulpal nociception was induced by intradental application of capsaicin (100 μg/rat). α-pinene (0.1, 0.2, and 0.4 μg/rat) was injected centrally 10 min before the administration of capsaicin. In addition, α-pinene (0.4 μg/rat) was co-injected with bicuculline (0.5 μg/rat). Spatial and passive avoidance learning and memory were assessed using Morris water maze (MWM) and shuttle box tasks, respectively. RESULTS: Experimental results of the MWM test showed that capsaicin increases escape latency and distance traveled to the hidden platform (P < 0.01). The effect was prohibited by α-pinene at the dose of 0.4 μg/rat. Moreover, capsaicin-treated animals spent less time in the target zone than capsaicin + α-pinene (0.4 μg/rat)-treated rats (P < 0.05). In the shuttle box test, α-pinene (0.2 μg and 0.4 μg) prevented an increased number of acquisition trials and time spent in the dark chamber induced by capsaicin, whereas it increased step-through latency (P < 0.01). However, the effects of α-pinene (0.4 μg/rat) in both tests were prohibited by bicuculline (0.5 μg/rat). CONCLUSION: The data showed that central administration of α-pinene might reduce pulpalgia-induced learning and memory impairment, at least partially, via modulation of GABA(A) receptors.
format Online
Article
Text
id pubmed-9482378
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-94823782022-09-18 α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor Rafie, Forouzan Kooshki, Razieh Abbasnejad, Mehdi Rahbar, Iran Raoof, Maryam Nekouei, Amir Hossein Adv Biomed Res Original Article BACKGROUND: This study investigated the effect of central administration of α-pinene and the interaction of α-pinene with GABAA receptor on pulpal nociception-induced changes in learning and memory performances in rats. MATERIALS AND METHODS: Sixty-six adult male Wistar rats were used. Pulpal nociception was induced by intradental application of capsaicin (100 μg/rat). α-pinene (0.1, 0.2, and 0.4 μg/rat) was injected centrally 10 min before the administration of capsaicin. In addition, α-pinene (0.4 μg/rat) was co-injected with bicuculline (0.5 μg/rat). Spatial and passive avoidance learning and memory were assessed using Morris water maze (MWM) and shuttle box tasks, respectively. RESULTS: Experimental results of the MWM test showed that capsaicin increases escape latency and distance traveled to the hidden platform (P < 0.01). The effect was prohibited by α-pinene at the dose of 0.4 μg/rat. Moreover, capsaicin-treated animals spent less time in the target zone than capsaicin + α-pinene (0.4 μg/rat)-treated rats (P < 0.05). In the shuttle box test, α-pinene (0.2 μg and 0.4 μg) prevented an increased number of acquisition trials and time spent in the dark chamber induced by capsaicin, whereas it increased step-through latency (P < 0.01). However, the effects of α-pinene (0.4 μg/rat) in both tests were prohibited by bicuculline (0.5 μg/rat). CONCLUSION: The data showed that central administration of α-pinene might reduce pulpalgia-induced learning and memory impairment, at least partially, via modulation of GABA(A) receptors. Wolters Kluwer - Medknow 2022-07-29 /pmc/articles/PMC9482378/ /pubmed/36124022 http://dx.doi.org/10.4103/abr.abr_139_21 Text en Copyright: © 2022 Advanced Biomedical Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Rafie, Forouzan
Kooshki, Razieh
Abbasnejad, Mehdi
Rahbar, Iran
Raoof, Maryam
Nekouei, Amir Hossein
α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title_full α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title_fullStr α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title_full_unstemmed α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title_short α-Pinene Influence on Pulpal Pain-Induced Learning and Memory Impairment in Rats Via Modulation of the GABAA Receptor
title_sort α-pinene influence on pulpal pain-induced learning and memory impairment in rats via modulation of the gabaa receptor
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482378/
https://www.ncbi.nlm.nih.gov/pubmed/36124022
http://dx.doi.org/10.4103/abr.abr_139_21
work_keys_str_mv AT rafieforouzan apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor
AT kooshkirazieh apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor
AT abbasnejadmehdi apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor
AT rahbariran apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor
AT raoofmaryam apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor
AT nekoueiamirhossein apineneinfluenceonpulpalpaininducedlearningandmemoryimpairmentinratsviamodulationofthegabaareceptor