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Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay

BACKGROUND: Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens. METHODS: RT-RAA-Cas12a-mediated real-time or end-point fluorescent and lateral flow strip (LFS) assay...

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Detalles Bibliográficos
Autores principales: Qian, Weidong, Huang, Jie, Wang, Ting, Fan, Cheng, Kang, Jie, Zhang, Qian, Li, Yongdong, Chen, Si
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482751/
https://www.ncbi.nlm.nih.gov/pubmed/36115975
http://dx.doi.org/10.1186/s12985-022-01878-z

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