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Ultrasensitive and visual detection of human norovirus genotype GII.4 or GII.17 using CRISPR-Cas12a assay
BACKGROUND: Integrating CRISPR-Cas12a sensors with isothermal signal amplification can be exploited to develop low-cost, disposable, and ultrasensitive assays for the diagnostics of human pathogens. METHODS: RT-RAA-Cas12a-mediated real-time or end-point fluorescent and lateral flow strip (LFS) assay...
Autores principales: | Qian, Weidong, Huang, Jie, Wang, Ting, Fan, Cheng, Kang, Jie, Zhang, Qian, Li, Yongdong, Chen, Si |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482751/ https://www.ncbi.nlm.nih.gov/pubmed/36115975 http://dx.doi.org/10.1186/s12985-022-01878-z |
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