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CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells
The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482892/ https://www.ncbi.nlm.nih.gov/pubmed/36117192 http://dx.doi.org/10.1186/s13619-022-00131-w |
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author | Gong, Shixin Hu, Gongcheng Guo, Rong Zhang, Jie Yang, Yiqi Ji, Binrui Li, Gang Yao, Hongjie |
author_facet | Gong, Shixin Hu, Gongcheng Guo, Rong Zhang, Jie Yang, Yiqi Ji, Binrui Li, Gang Yao, Hongjie |
author_sort | Gong, Shixin |
collection | PubMed |
description | The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 is required for the CTCF interaction with CBP. A CTCF point mutation at lysine 20 had no effect on self-renewal but blocked the mesoderm differentiation of mouse embryonic stem cells (mESCs). The CTCF-K20 mutation reduced CTCF binding to the promoters and enhancers of genes associated with early cardiac mesoderm differentiation, resulting in diminished chromatin accessibility and decreased enhancer-promoter interactions, impairing gene expression. In summary, this study reveals the important roles of CTCF-K20 in regulating CTCF genomic functions and mESC differentiation into mesoderm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-022-00131-w. |
format | Online Article Text |
id | pubmed-9482892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-94828922022-09-30 CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells Gong, Shixin Hu, Gongcheng Guo, Rong Zhang, Jie Yang, Yiqi Ji, Binrui Li, Gang Yao, Hongjie Cell Regen Research Article The CCCTC-binding factor (CTCF) protein and its modified forms regulate gene expression and genome organization. However, information on CTCF acetylation and its biological function is still lacking. Here, we show that CTCF can be acetylated at lysine 20 (CTCF-K20) by CREB-binding protein (CBP) and deacetylated by histone deacetylase 6 (HDAC6). CTCF-K20 is required for the CTCF interaction with CBP. A CTCF point mutation at lysine 20 had no effect on self-renewal but blocked the mesoderm differentiation of mouse embryonic stem cells (mESCs). The CTCF-K20 mutation reduced CTCF binding to the promoters and enhancers of genes associated with early cardiac mesoderm differentiation, resulting in diminished chromatin accessibility and decreased enhancer-promoter interactions, impairing gene expression. In summary, this study reveals the important roles of CTCF-K20 in regulating CTCF genomic functions and mESC differentiation into mesoderm. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13619-022-00131-w. Springer Nature Singapore 2022-09-19 /pmc/articles/PMC9482892/ /pubmed/36117192 http://dx.doi.org/10.1186/s13619-022-00131-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Gong, Shixin Hu, Gongcheng Guo, Rong Zhang, Jie Yang, Yiqi Ji, Binrui Li, Gang Yao, Hongjie CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title_full | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title_fullStr | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title_full_unstemmed | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title_short | CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
title_sort | ctcf acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482892/ https://www.ncbi.nlm.nih.gov/pubmed/36117192 http://dx.doi.org/10.1186/s13619-022-00131-w |
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