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Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification

The Burkholderia cepacia complex (BCC) is a group of opportunistic pathogens, including Burkholderia cepacia, Burkholderia multivorans, Burkholderia vietnamiensis and Burkholderia ambifaria, which can cause severe respiratory tract infections and lead to high mortality rates among humans. The early...

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Autores principales: Fu, Hanyu, Gan, Lin, Tian, Ziyan, Han, Juqiang, Du, Bing, Xue, Guanhua, Feng, Yanling, Zhao, Hanqing, Cui, Jinghua, Yan, Chao, Feng, Junxia, Fan, Zheng, Fu, Tongtong, Xu, Ziying, Zhang, Rui, Cui, Xiaohu, Du, Shuheng, Zhou, Yao, Zhang, Qun, Cao, Ling, Yuan, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483118/
https://www.ncbi.nlm.nih.gov/pubmed/36132989
http://dx.doi.org/10.3389/fcimb.2022.984140
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author Fu, Hanyu
Gan, Lin
Tian, Ziyan
Han, Juqiang
Du, Bing
Xue, Guanhua
Feng, Yanling
Zhao, Hanqing
Cui, Jinghua
Yan, Chao
Feng, Junxia
Fan, Zheng
Fu, Tongtong
Xu, Ziying
Zhang, Rui
Cui, Xiaohu
Du, Shuheng
Zhou, Yao
Zhang, Qun
Cao, Ling
Yuan, Jing
author_facet Fu, Hanyu
Gan, Lin
Tian, Ziyan
Han, Juqiang
Du, Bing
Xue, Guanhua
Feng, Yanling
Zhao, Hanqing
Cui, Jinghua
Yan, Chao
Feng, Junxia
Fan, Zheng
Fu, Tongtong
Xu, Ziying
Zhang, Rui
Cui, Xiaohu
Du, Shuheng
Zhou, Yao
Zhang, Qun
Cao, Ling
Yuan, Jing
author_sort Fu, Hanyu
collection PubMed
description The Burkholderia cepacia complex (BCC) is a group of opportunistic pathogens, including Burkholderia cepacia, Burkholderia multivorans, Burkholderia vietnamiensis and Burkholderia ambifaria, which can cause severe respiratory tract infections and lead to high mortality rates among humans. The early diagnosis and effective treatment of BCC infection are therefore crucial. In this study, a novel and rapid recombinase-aided amplification (RAA) assay targeting the 16S rRNA gene was developed for BCC detection. The protocol for this RAA assay could be completed in 10 min at 39°C, with a sensitivity of 10 copies per reaction and no cross-reactivity with other pathogens. To characterize the effectiveness of the RAA assay, we further collected 269 clinical samples from patients with bacterial pneumonia. The sensitivity and specificity of the RAA assay were 100% and 98.5%, respectively. Seven BCC-infected patients were detected using the RAA assay, and three BCC strains were isolated from the 269 clinical samples. Our data showed that the prevalence of BCC infection was 2.60%, which is higher than the 1.40% reported in previous studies, suggesting that high sensitivity is vital to BCC detection. We also screened a patient with B. vietnamiensis infection using the RAA assay in clinic, allowing for appropriate treatment to be initiated rapidly. Together, these data indicate that the RAA assay targeting the 16S rRNA gene can be applied for the early and rapid detection of BCC pathogens in patients with an uncharacterized infection who are immunocompromised or have underlying diseases, thereby providing guidance for effective treatment.
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spelling pubmed-94831182022-09-20 Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification Fu, Hanyu Gan, Lin Tian, Ziyan Han, Juqiang Du, Bing Xue, Guanhua Feng, Yanling Zhao, Hanqing Cui, Jinghua Yan, Chao Feng, Junxia Fan, Zheng Fu, Tongtong Xu, Ziying Zhang, Rui Cui, Xiaohu Du, Shuheng Zhou, Yao Zhang, Qun Cao, Ling Yuan, Jing Front Cell Infect Microbiol Cellular and Infection Microbiology The Burkholderia cepacia complex (BCC) is a group of opportunistic pathogens, including Burkholderia cepacia, Burkholderia multivorans, Burkholderia vietnamiensis and Burkholderia ambifaria, which can cause severe respiratory tract infections and lead to high mortality rates among humans. The early diagnosis and effective treatment of BCC infection are therefore crucial. In this study, a novel and rapid recombinase-aided amplification (RAA) assay targeting the 16S rRNA gene was developed for BCC detection. The protocol for this RAA assay could be completed in 10 min at 39°C, with a sensitivity of 10 copies per reaction and no cross-reactivity with other pathogens. To characterize the effectiveness of the RAA assay, we further collected 269 clinical samples from patients with bacterial pneumonia. The sensitivity and specificity of the RAA assay were 100% and 98.5%, respectively. Seven BCC-infected patients were detected using the RAA assay, and three BCC strains were isolated from the 269 clinical samples. Our data showed that the prevalence of BCC infection was 2.60%, which is higher than the 1.40% reported in previous studies, suggesting that high sensitivity is vital to BCC detection. We also screened a patient with B. vietnamiensis infection using the RAA assay in clinic, allowing for appropriate treatment to be initiated rapidly. Together, these data indicate that the RAA assay targeting the 16S rRNA gene can be applied for the early and rapid detection of BCC pathogens in patients with an uncharacterized infection who are immunocompromised or have underlying diseases, thereby providing guidance for effective treatment. Frontiers Media S.A. 2022-09-05 /pmc/articles/PMC9483118/ /pubmed/36132989 http://dx.doi.org/10.3389/fcimb.2022.984140 Text en Copyright © 2022 Fu, Gan, Tian, Han, Du, Xue, Feng, Zhao, Cui, Yan, Feng, Fan, Fu, Xu, Zhang, Cui, Du, Zhou, Zhang, Cao and Yuan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Fu, Hanyu
Gan, Lin
Tian, Ziyan
Han, Juqiang
Du, Bing
Xue, Guanhua
Feng, Yanling
Zhao, Hanqing
Cui, Jinghua
Yan, Chao
Feng, Junxia
Fan, Zheng
Fu, Tongtong
Xu, Ziying
Zhang, Rui
Cui, Xiaohu
Du, Shuheng
Zhou, Yao
Zhang, Qun
Cao, Ling
Yuan, Jing
Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title_full Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title_fullStr Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title_full_unstemmed Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title_short Rapid detection of Burkholderia cepacia complex carrying the 16S rRNA gene in clinical specimens by recombinase-aided amplification
title_sort rapid detection of burkholderia cepacia complex carrying the 16s rrna gene in clinical specimens by recombinase-aided amplification
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483118/
https://www.ncbi.nlm.nih.gov/pubmed/36132989
http://dx.doi.org/10.3389/fcimb.2022.984140
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