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The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration

Vimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscl...

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Autores principales: Soglia, Francesca, Bordini, Martina, Mazzoni, Maurizio, Zappaterra, Martina, Di Nunzio, Mattia, Clavenzani, Paolo, Davoli, Roberta, Meluzzi, Adele, Sirri, Federico, Petracci, Massimiliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483144/
https://www.ncbi.nlm.nih.gov/pubmed/36134328
http://dx.doi.org/10.3389/fphys.2022.970034
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author Soglia, Francesca
Bordini, Martina
Mazzoni, Maurizio
Zappaterra, Martina
Di Nunzio, Mattia
Clavenzani, Paolo
Davoli, Roberta
Meluzzi, Adele
Sirri, Federico
Petracci, Massimiliano
author_facet Soglia, Francesca
Bordini, Martina
Mazzoni, Maurizio
Zappaterra, Martina
Di Nunzio, Mattia
Clavenzani, Paolo
Davoli, Roberta
Meluzzi, Adele
Sirri, Federico
Petracci, Massimiliano
author_sort Soglia, Francesca
collection PubMed
description Vimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscles (PM) of fast-growing (FG) and medium-growing (MG) meat-type chickens both at the protein and gene levels. MG broilers were considered as a control group whereas the evolution of VIM and DES over the growth period was evaluated in FG by collecting samples at different developmental stages (7, 14, 21, 28, 35, and 42 days). After performing a preliminary classification of the samples based on their histological features, 5 PM/sampling time/genotype were selected for western blot, immunohistochemistry (IHC), and gene expression analyses. Overall, the findings obtained at the protein level mirrored those related to their encoding genes, although a potential time lag required to observe the consequences of gene expression was evident. The two- and 3-fold higher level of the VIM-based heterodimer observed in FG at d 21 and d 28 in comparison with MG of the same age might be ascribed to the beginning and progressive development of the regenerative processes. This hypothesis is supported by IHC highlighting the presence of fibers to co-expressing VIM and DES. In addition, gene expression analyses suggested that, unlike VIM common sequence, VIM long isoform may not be directly implicated in muscle regeneration. As for DES content, the fluctuating trends observed for both the native protein and its heterodimer in FG might be ascribed to its importance for maintaining the structural organization of the regenerating fibers. Furthermore, the higher expression level of the DES gene in FG in comparison with MG further supported its potential application as a marker of muscle fibers’ regeneration. In conclusion, the findings of the present research seem to support the existence of a relationship between the occurrence of muscle regeneration and the growth rate of meat-type chickens and corroborate the potential use of VIM and DES as molecular markers of these cellular processes.
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spelling pubmed-94831442022-09-20 The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration Soglia, Francesca Bordini, Martina Mazzoni, Maurizio Zappaterra, Martina Di Nunzio, Mattia Clavenzani, Paolo Davoli, Roberta Meluzzi, Adele Sirri, Federico Petracci, Massimiliano Front Physiol Physiology Vimentin (VIM) and desmin (DES) are muscle-specific proteins having crucial roles in maintaining the lateral organization and alignment of the sarcomeric structure during myofibrils’ regeneration. The present experiment was designed to ascertain the evolution of VIM and DES in Pectoralis major muscles (PM) of fast-growing (FG) and medium-growing (MG) meat-type chickens both at the protein and gene levels. MG broilers were considered as a control group whereas the evolution of VIM and DES over the growth period was evaluated in FG by collecting samples at different developmental stages (7, 14, 21, 28, 35, and 42 days). After performing a preliminary classification of the samples based on their histological features, 5 PM/sampling time/genotype were selected for western blot, immunohistochemistry (IHC), and gene expression analyses. Overall, the findings obtained at the protein level mirrored those related to their encoding genes, although a potential time lag required to observe the consequences of gene expression was evident. The two- and 3-fold higher level of the VIM-based heterodimer observed in FG at d 21 and d 28 in comparison with MG of the same age might be ascribed to the beginning and progressive development of the regenerative processes. This hypothesis is supported by IHC highlighting the presence of fibers to co-expressing VIM and DES. In addition, gene expression analyses suggested that, unlike VIM common sequence, VIM long isoform may not be directly implicated in muscle regeneration. As for DES content, the fluctuating trends observed for both the native protein and its heterodimer in FG might be ascribed to its importance for maintaining the structural organization of the regenerating fibers. Furthermore, the higher expression level of the DES gene in FG in comparison with MG further supported its potential application as a marker of muscle fibers’ regeneration. In conclusion, the findings of the present research seem to support the existence of a relationship between the occurrence of muscle regeneration and the growth rate of meat-type chickens and corroborate the potential use of VIM and DES as molecular markers of these cellular processes. Frontiers Media S.A. 2022-09-05 /pmc/articles/PMC9483144/ /pubmed/36134328 http://dx.doi.org/10.3389/fphys.2022.970034 Text en Copyright © 2022 Soglia, Bordini, Mazzoni, Zappaterra, Di Nunzio, Clavenzani, Davoli, Meluzzi, Sirri and Petracci. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Soglia, Francesca
Bordini, Martina
Mazzoni, Maurizio
Zappaterra, Martina
Di Nunzio, Mattia
Clavenzani, Paolo
Davoli, Roberta
Meluzzi, Adele
Sirri, Federico
Petracci, Massimiliano
The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title_full The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title_fullStr The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title_full_unstemmed The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title_short The evolution of vimentin and desmin in Pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
title_sort evolution of vimentin and desmin in pectoralis major muscles of broiler chickens supports their essential role in muscle regeneration
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483144/
https://www.ncbi.nlm.nih.gov/pubmed/36134328
http://dx.doi.org/10.3389/fphys.2022.970034
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