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Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome

Coiled coils (CCs) are protein structural motifs universally found in proteins and mediate a plethora of biological interactions, and thus their reliable annotation is crucial for studies of protein structure and function. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a large doub...

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Autores principales: Qiu, Jianxiang, Liu, Zumei, Fang, Zhixin, Wu, Wenjiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483598/
https://www.ncbi.nlm.nih.gov/pubmed/36132175
http://dx.doi.org/10.1016/j.heliyon.2022.e10588
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author Qiu, Jianxiang
Liu, Zumei
Fang, Zhixin
Wu, Wenjiao
author_facet Qiu, Jianxiang
Liu, Zumei
Fang, Zhixin
Wu, Wenjiao
author_sort Qiu, Jianxiang
collection PubMed
description Coiled coils (CCs) are protein structural motifs universally found in proteins and mediate a plethora of biological interactions, and thus their reliable annotation is crucial for studies of protein structure and function. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a large double-stranded DNA (dsDNA) virus and encodes 154 proteins. In this study, genome-wide scans of previously uncharacterized CC motifs throughout AcMNPV was conducted using CC prediction software. In total, 24 CC motifs in 19 CC proteins with high confidence were identified. The characteristic of viral CC motifs were analyzed. The CC proteins could be divided into 12 viral structural proteins and 7 non-structural proteins, including viral membrane fusion proteins, enzymes, and transcription factors. Moreover, CC motifs are conserved in the baculoviral orthologs of 14 of the 19 proteins. It is noted that five CC proteins, including Ac51, Ac66, Exon0, Ac13, and GP16, were previously identified to function in the nuclear egress of nucleocapsids, and Ac66 contains multiple CC motifs, the longest of which comprises 252 amino acids, suggesting a role of CC motifs in this process. Taken together, the CC motifs identified in this study are valuable resource for studying protein function and protein interaction networks during virus replication.
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spelling pubmed-94835982022-09-20 Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome Qiu, Jianxiang Liu, Zumei Fang, Zhixin Wu, Wenjiao Heliyon Research Article Coiled coils (CCs) are protein structural motifs universally found in proteins and mediate a plethora of biological interactions, and thus their reliable annotation is crucial for studies of protein structure and function. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a large double-stranded DNA (dsDNA) virus and encodes 154 proteins. In this study, genome-wide scans of previously uncharacterized CC motifs throughout AcMNPV was conducted using CC prediction software. In total, 24 CC motifs in 19 CC proteins with high confidence were identified. The characteristic of viral CC motifs were analyzed. The CC proteins could be divided into 12 viral structural proteins and 7 non-structural proteins, including viral membrane fusion proteins, enzymes, and transcription factors. Moreover, CC motifs are conserved in the baculoviral orthologs of 14 of the 19 proteins. It is noted that five CC proteins, including Ac51, Ac66, Exon0, Ac13, and GP16, were previously identified to function in the nuclear egress of nucleocapsids, and Ac66 contains multiple CC motifs, the longest of which comprises 252 amino acids, suggesting a role of CC motifs in this process. Taken together, the CC motifs identified in this study are valuable resource for studying protein function and protein interaction networks during virus replication. Elsevier 2022-09-10 /pmc/articles/PMC9483598/ /pubmed/36132175 http://dx.doi.org/10.1016/j.heliyon.2022.e10588 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Qiu, Jianxiang
Liu, Zumei
Fang, Zhixin
Wu, Wenjiao
Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title_full Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title_fullStr Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title_full_unstemmed Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title_short Identification and characterization of coiled-coil motifs across Autographa californica multiple nucleopolyhedrovirus genome
title_sort identification and characterization of coiled-coil motifs across autographa californica multiple nucleopolyhedrovirus genome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483598/
https://www.ncbi.nlm.nih.gov/pubmed/36132175
http://dx.doi.org/10.1016/j.heliyon.2022.e10588
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