Cargando…
Optimized protocol to isolate primary mouse peritoneal macrophage metabolites
Peritoneal macrophages (PMs) have been shown to have higher stability compared to other macrophage subtypes. However, obtaining enough PMs from a single mouse is often a limitation for metabolomics analysis. Here, we describe a protocol to isolate metabolites from a small number of mouse primary PMs...
Autores principales: | De Jesus, Adam, Pusec, Carolina M., Nguyen, Tivoli, Keyhani-Nejad, Farnaz, Gao, Peng, Weinberg, Samuel E., Ardehali, Hossein |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483642/ https://www.ncbi.nlm.nih.gov/pubmed/36103306 http://dx.doi.org/10.1016/j.xpro.2022.101668 |
Ejemplares similares
-
A protocol for macrophage depletion and reconstitution in a mouse model of sepsis
por: Nguyen, Tivoli, et al.
Publicado: (2021) -
Optimized protocol for quantification of mitochondrial non-heme and heme iron content in mouse tissues and cultured cells
por: Sato, Tatsuya, et al.
Publicado: (2023) -
Liver-specific overexpression of HKDC1 increases hepatocyte size and proliferative capacity
por: Pusec, Carolina M., et al.
Publicado: (2023) -
Protocol for isolation and analysis of small volatile microbiome metabolites from human or mouse samples
por: Bell, Hannah N., et al.
Publicado: (2022) -
Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
por: Ferriz, Margarita, et al.
Publicado: (2023)