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Optimized protocol to isolate primary mouse peritoneal macrophage metabolites

Peritoneal macrophages (PMs) have been shown to have higher stability compared to other macrophage subtypes. However, obtaining enough PMs from a single mouse is often a limitation for metabolomics analysis. Here, we describe a protocol to isolate metabolites from a small number of mouse primary PMs...

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Detalles Bibliográficos
Autores principales: De Jesus, Adam, Pusec, Carolina M., Nguyen, Tivoli, Keyhani-Nejad, Farnaz, Gao, Peng, Weinberg, Samuel E., Ardehali, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483642/
https://www.ncbi.nlm.nih.gov/pubmed/36103306
http://dx.doi.org/10.1016/j.xpro.2022.101668

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