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Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma

OBJECTIVE: To investigate the prevalence of p53 mutations and associated factors between immunohistochemistry (IHC) and p53 staining patterns among patients with high-grade serous ovarian carcinoma (HGSOC). METHODS: This study is a retrospective review. A total of 62 patients with HGSOC underwent su...

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Autores principales: Orachum, Panarat, Temtanakitpaisan, Amornrat, Kleebkaow, Pilaiwan, Chumworathayi, Bandit, Luanratanakorn, Sanguanchoke, Aue-angkul, Apiwat, Itarat, Yuwadee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Obstetrics and Gynecology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483669/
https://www.ncbi.nlm.nih.gov/pubmed/35903943
http://dx.doi.org/10.5468/ogs.22102
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author Orachum, Panarat
Temtanakitpaisan, Amornrat
Kleebkaow, Pilaiwan
Chumworathayi, Bandit
Luanratanakorn, Sanguanchoke
Aue-angkul, Apiwat
Itarat, Yuwadee
author_facet Orachum, Panarat
Temtanakitpaisan, Amornrat
Kleebkaow, Pilaiwan
Chumworathayi, Bandit
Luanratanakorn, Sanguanchoke
Aue-angkul, Apiwat
Itarat, Yuwadee
author_sort Orachum, Panarat
collection PubMed
description OBJECTIVE: To investigate the prevalence of p53 mutations and associated factors between immunohistochemistry (IHC) and p53 staining patterns among patients with high-grade serous ovarian carcinoma (HGSOC). METHODS: This study is a retrospective review. A total of 62 patients with HGSOC underwent surgery at Srinagarind Hospital between January 2016 and December 2020. Histological examination was performed based on a combination of morphology and IHC staining with p53. The p53 immunostaining pattern was interpreted as a missense mutation, nonsense mutation, or a wild-type pattern. Missense (p53 overexpression pattern) and nonsense (null expression p53 pattern) mutations were considered p53 mutations. A wild-type pattern was defined as a p53 non-mutation. RESULTS: p53 mutations were identified in 93.6% of the patients. Subgroup analysis of the p53 mutation group between the p53 overexpression pattern and the p53 null expression pattern in terms of clinicopathological characteristics and initial treatment was performed. Patients with the p53 overexpression pattern had significantly more omental metastases than those with the p53 null expression pattern (87.8% vs. 64.7%, P=0.042). There were no statistically significant differences in median progression-free survival (PFS) (9 vs. 10 months, P=0.813) or median overall survival (OS) (12 vs. 17 months, P=0.526) between the two groups. CONCLUSION: The prevalence of p53 mutations in HGSOC patients in this study was 93.6%. Omental metastasis is a significant pathological factor in predicting overexpression p53 pattern in HGSC. However, IHC analysis of the p53 staining pattern did not affect OS or PFS among patients with HGSOC.
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spelling pubmed-94836692022-09-22 Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma Orachum, Panarat Temtanakitpaisan, Amornrat Kleebkaow, Pilaiwan Chumworathayi, Bandit Luanratanakorn, Sanguanchoke Aue-angkul, Apiwat Itarat, Yuwadee Obstet Gynecol Sci Original Article OBJECTIVE: To investigate the prevalence of p53 mutations and associated factors between immunohistochemistry (IHC) and p53 staining patterns among patients with high-grade serous ovarian carcinoma (HGSOC). METHODS: This study is a retrospective review. A total of 62 patients with HGSOC underwent surgery at Srinagarind Hospital between January 2016 and December 2020. Histological examination was performed based on a combination of morphology and IHC staining with p53. The p53 immunostaining pattern was interpreted as a missense mutation, nonsense mutation, or a wild-type pattern. Missense (p53 overexpression pattern) and nonsense (null expression p53 pattern) mutations were considered p53 mutations. A wild-type pattern was defined as a p53 non-mutation. RESULTS: p53 mutations were identified in 93.6% of the patients. Subgroup analysis of the p53 mutation group between the p53 overexpression pattern and the p53 null expression pattern in terms of clinicopathological characteristics and initial treatment was performed. Patients with the p53 overexpression pattern had significantly more omental metastases than those with the p53 null expression pattern (87.8% vs. 64.7%, P=0.042). There were no statistically significant differences in median progression-free survival (PFS) (9 vs. 10 months, P=0.813) or median overall survival (OS) (12 vs. 17 months, P=0.526) between the two groups. CONCLUSION: The prevalence of p53 mutations in HGSOC patients in this study was 93.6%. Omental metastasis is a significant pathological factor in predicting overexpression p53 pattern in HGSC. However, IHC analysis of the p53 staining pattern did not affect OS or PFS among patients with HGSOC. Korean Society of Obstetrics and Gynecology 2022-09 2022-07-29 /pmc/articles/PMC9483669/ /pubmed/35903943 http://dx.doi.org/10.5468/ogs.22102 Text en Copyright © 2022 Korean Society of Obstetrics and Gynecology https://creativecommons.org/licenses/by-nc/3.0/Articles published in Obstet Gynecol Sci are open-access, distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Orachum, Panarat
Temtanakitpaisan, Amornrat
Kleebkaow, Pilaiwan
Chumworathayi, Bandit
Luanratanakorn, Sanguanchoke
Aue-angkul, Apiwat
Itarat, Yuwadee
Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title_full Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title_fullStr Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title_full_unstemmed Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title_short Clinical outcomes of immunohistochemistry of the p53 Staining pattern in high-grade serous ovarian carcinoma
title_sort clinical outcomes of immunohistochemistry of the p53 staining pattern in high-grade serous ovarian carcinoma
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483669/
https://www.ncbi.nlm.nih.gov/pubmed/35903943
http://dx.doi.org/10.5468/ogs.22102
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