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Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis
Nontuberculous mycobacterial (NTM) infections are growing worldwide especially in immunocompromised individuals. Since treatment of NTM infections is species-specific, the precise identification of NTM to species level is critical for an optimal treatment. This study was aimed to identify different...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483868/ https://www.ncbi.nlm.nih.gov/pubmed/36121509 http://dx.doi.org/10.1186/s13568-022-01463-1 |
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author | Khosravi, Azar Dokht Hashemzadeh, Mohammad Rokhfirooz, Parisa |
author_facet | Khosravi, Azar Dokht Hashemzadeh, Mohammad Rokhfirooz, Parisa |
author_sort | Khosravi, Azar Dokht |
collection | PubMed |
description | Nontuberculous mycobacterial (NTM) infections are growing worldwide especially in immunocompromised individuals. Since treatment of NTM infections is species-specific, the precise identification of NTM to species level is critical for an optimal treatment. This study was aimed to identify different NTM species by sequencing the rpoB gene and evaluating the effectiveness of argH and cya gene markers. In total 64 clinical isolates suspected to NTM were collected. The identification of the isolates was done by standard conventional methods and PCR-based rpoB gene and sequence analysis. PCR sequencing of argH and cya genes was performed to evaluate the efficacy of these genes in identifying and differentiating different species and subspecies of NTM. Among 64 isolates tested, 51 (79.68%) were detected by conventional tests as NTM. The results of rpoB sequence analysis revealed that the 56 clinical isolates were identified in 10 species of NTM and 8 remaining isolates which showed ambiguous results by rpoB sequencing, application of argH and cya sequencing could detect these isolates. Furthermore, by using cya gene sequencing, M. abscessus subspecies were properly differentiated. Although the rpoB sequencing as a standard method, is beneficial for detecting various species of NTM, however, based on our findings, argH and cya gene markers have a superb ability to discriminate closely related species. Further investigations are required to verify our outcomes. |
format | Online Article Text |
id | pubmed-9483868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-94838682022-09-19 Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis Khosravi, Azar Dokht Hashemzadeh, Mohammad Rokhfirooz, Parisa AMB Express Original Article Nontuberculous mycobacterial (NTM) infections are growing worldwide especially in immunocompromised individuals. Since treatment of NTM infections is species-specific, the precise identification of NTM to species level is critical for an optimal treatment. This study was aimed to identify different NTM species by sequencing the rpoB gene and evaluating the effectiveness of argH and cya gene markers. In total 64 clinical isolates suspected to NTM were collected. The identification of the isolates was done by standard conventional methods and PCR-based rpoB gene and sequence analysis. PCR sequencing of argH and cya genes was performed to evaluate the efficacy of these genes in identifying and differentiating different species and subspecies of NTM. Among 64 isolates tested, 51 (79.68%) were detected by conventional tests as NTM. The results of rpoB sequence analysis revealed that the 56 clinical isolates were identified in 10 species of NTM and 8 remaining isolates which showed ambiguous results by rpoB sequencing, application of argH and cya sequencing could detect these isolates. Furthermore, by using cya gene sequencing, M. abscessus subspecies were properly differentiated. Although the rpoB sequencing as a standard method, is beneficial for detecting various species of NTM, however, based on our findings, argH and cya gene markers have a superb ability to discriminate closely related species. Further investigations are required to verify our outcomes. Springer Berlin Heidelberg 2022-09-19 /pmc/articles/PMC9483868/ /pubmed/36121509 http://dx.doi.org/10.1186/s13568-022-01463-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Khosravi, Azar Dokht Hashemzadeh, Mohammad Rokhfirooz, Parisa Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title | Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title_full | Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title_fullStr | Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title_full_unstemmed | Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title_short | Molecular identification of nontuberculous mycobacteria using the rpoB, argH and cya genes analysis |
title_sort | molecular identification of nontuberculous mycobacteria using the rpob, argh and cya genes analysis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483868/ https://www.ncbi.nlm.nih.gov/pubmed/36121509 http://dx.doi.org/10.1186/s13568-022-01463-1 |
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