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Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484135/ https://www.ncbi.nlm.nih.gov/pubmed/36155953 http://dx.doi.org/10.1016/j.bios.2022.114739 |
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author | Meng, Xiangqin Zou, Sijia Li, Dandan He, Jiuyang Fang, Ling Wang, Haojue Yan, Xiyun Duan, Demin Gao, Lizeng |
author_facet | Meng, Xiangqin Zou, Sijia Li, Dandan He, Jiuyang Fang, Ling Wang, Haojue Yan, Xiyun Duan, Demin Gao, Lizeng |
author_sort | Meng, Xiangqin |
collection | PubMed |
description | The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS(2) nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS(2) nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS(2) nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections. |
format | Online Article Text |
id | pubmed-9484135 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94841352022-09-19 Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 Meng, Xiangqin Zou, Sijia Li, Dandan He, Jiuyang Fang, Ling Wang, Haojue Yan, Xiyun Duan, Demin Gao, Lizeng Biosens Bioelectron Article The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS(2) nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS(2) nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS(2) nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections. Elsevier B.V. 2022-12-01 2022-09-19 /pmc/articles/PMC9484135/ /pubmed/36155953 http://dx.doi.org/10.1016/j.bios.2022.114739 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Meng, Xiangqin Zou, Sijia Li, Dandan He, Jiuyang Fang, Ling Wang, Haojue Yan, Xiyun Duan, Demin Gao, Lizeng Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title | Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title_full | Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title_fullStr | Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title_full_unstemmed | Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title_short | Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2 |
title_sort | nanozyme-strip for rapid and ultrasensitive nucleic acid detection of sars-cov-2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484135/ https://www.ncbi.nlm.nih.gov/pubmed/36155953 http://dx.doi.org/10.1016/j.bios.2022.114739 |
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