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Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS

Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assiste...

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Autores principales: Teixeira, Diego Oliveira, Silva, Herlon Victor Rodrigues, Brito, Bruna Farias, Barbosa, Brenna de Sousa, Tabosa, Beatriz Evaristo de Almeida, da Silva, Lúcia Daniel Machado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484261/
https://www.ncbi.nlm.nih.gov/pubmed/36156885
http://dx.doi.org/10.1590/1984-3143-AR2021-0069
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author Teixeira, Diego Oliveira
Silva, Herlon Victor Rodrigues
Brito, Bruna Farias
Barbosa, Brenna de Sousa
Tabosa, Beatriz Evaristo de Almeida
da Silva, Lúcia Daniel Machado
author_facet Teixeira, Diego Oliveira
Silva, Herlon Victor Rodrigues
Brito, Bruna Farias
Barbosa, Brenna de Sousa
Tabosa, Beatriz Evaristo de Almeida
da Silva, Lúcia Daniel Machado
author_sort Teixeira, Diego Oliveira
collection PubMed
description Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.
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spelling pubmed-94842612022-09-23 Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS Teixeira, Diego Oliveira Silva, Herlon Victor Rodrigues Brito, Bruna Farias Barbosa, Brenna de Sousa Tabosa, Beatriz Evaristo de Almeida da Silva, Lúcia Daniel Machado Anim Reprod Original Article Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP-106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing. Colégio Brasileiro de Reprodução Animal 2022-09-05 /pmc/articles/PMC9484261/ /pubmed/36156885 http://dx.doi.org/10.1590/1984-3143-AR2021-0069 Text en https://creativecommons.org/licenses/by/4.0/Copyright © The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Teixeira, Diego Oliveira
Silva, Herlon Victor Rodrigues
Brito, Bruna Farias
Barbosa, Brenna de Sousa
Tabosa, Beatriz Evaristo de Almeida
da Silva, Lúcia Daniel Machado
Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title_full Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title_fullStr Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title_full_unstemmed Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title_short Sperm quality and morphometry characterization of cryopreserved canine sperm in ACP-106c or TRIS
title_sort sperm quality and morphometry characterization of cryopreserved canine sperm in acp-106c or tris
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484261/
https://www.ncbi.nlm.nih.gov/pubmed/36156885
http://dx.doi.org/10.1590/1984-3143-AR2021-0069
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