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Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype
BACKGROUNDS: Chronic stress could induce depression-like phenotype in animal models. Previous data showed that sex differences exist after chronic stress model establishment, however, the detailed information about the difference of blood biochemical indexes is not clear. In this study, we aim to su...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484450/ https://www.ncbi.nlm.nih.gov/pubmed/36132219 http://dx.doi.org/10.7717/peerj.14014 |
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author | Chen, Yitian Cai, Weijia Li, Canye Su, Zuanjun Guo, Zhijun Li, Zhuman Wang, Chen Xu, Feng |
author_facet | Chen, Yitian Cai, Weijia Li, Canye Su, Zuanjun Guo, Zhijun Li, Zhuman Wang, Chen Xu, Feng |
author_sort | Chen, Yitian |
collection | PubMed |
description | BACKGROUNDS: Chronic stress could induce depression-like phenotype in animal models. Previous data showed that sex differences exist after chronic stress model establishment, however, the detailed information about the difference of blood biochemical indexes is not clear. In this study, we aim to supply comparison of monoamine transmitters and related hormone markers in serum between male and female depressed mice, and in order to better understand the sex difference in transmitters and hormone levels in depression occurrence and development. METHODS: Sixty C57BL/6 mice (both male and female) were divided into two groups by gender. Same gender mice were then divided randomly into the non-treated control group and chronic stress group which was exposed to 8 weeks of chronic unpredictable mild stress (CUMS). Depression-like behavior was assessed with open-field test and sucrose preference test. Blood sample was collected and monoamine transmitter and related hormone in serum were measured by ELISA. RESULTS: The depression-like phenotype mice model was established successfully after 8 weeks of chronic stress. The locomotion activity scores in male stressed mice declined more than that in female stressed mice, while the exploratory behavior scores in female stressed mice declined more than that in male stressed mice. Compared to non-treated control group mice, mice in the chronic stress group in response to stress showed greater declines in monoamine transmitters (5-HT, dopamine, norepinephrine) and sex hormones (androgen, estrogen, oxytocin and prolactin), while stress hormones (adrenaline, corticosterone and ACTH) were significantly increased. The decrease of norepinephrine, androgen and estrogen in female stressed mice was greater than in male stressed mice, whereas the 5-HT and oxytocin in male stressed mice decreased more than in female stressed mice, and the corticosterone in male stressed mice increased more than in female stressed mice. CONCLUSION: Sex differences of monoamine transmitter and related hormone levels in serum occurred in chronic stress induced depression-like phenotype mice model. It may provide a useful reference to guide precise antidepressant treatment in different gender population in clinical care. |
format | Online Article Text |
id | pubmed-9484450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94844502022-09-20 Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype Chen, Yitian Cai, Weijia Li, Canye Su, Zuanjun Guo, Zhijun Li, Zhuman Wang, Chen Xu, Feng PeerJ Animal Behavior BACKGROUNDS: Chronic stress could induce depression-like phenotype in animal models. Previous data showed that sex differences exist after chronic stress model establishment, however, the detailed information about the difference of blood biochemical indexes is not clear. In this study, we aim to supply comparison of monoamine transmitters and related hormone markers in serum between male and female depressed mice, and in order to better understand the sex difference in transmitters and hormone levels in depression occurrence and development. METHODS: Sixty C57BL/6 mice (both male and female) were divided into two groups by gender. Same gender mice were then divided randomly into the non-treated control group and chronic stress group which was exposed to 8 weeks of chronic unpredictable mild stress (CUMS). Depression-like behavior was assessed with open-field test and sucrose preference test. Blood sample was collected and monoamine transmitter and related hormone in serum were measured by ELISA. RESULTS: The depression-like phenotype mice model was established successfully after 8 weeks of chronic stress. The locomotion activity scores in male stressed mice declined more than that in female stressed mice, while the exploratory behavior scores in female stressed mice declined more than that in male stressed mice. Compared to non-treated control group mice, mice in the chronic stress group in response to stress showed greater declines in monoamine transmitters (5-HT, dopamine, norepinephrine) and sex hormones (androgen, estrogen, oxytocin and prolactin), while stress hormones (adrenaline, corticosterone and ACTH) were significantly increased. The decrease of norepinephrine, androgen and estrogen in female stressed mice was greater than in male stressed mice, whereas the 5-HT and oxytocin in male stressed mice decreased more than in female stressed mice, and the corticosterone in male stressed mice increased more than in female stressed mice. CONCLUSION: Sex differences of monoamine transmitter and related hormone levels in serum occurred in chronic stress induced depression-like phenotype mice model. It may provide a useful reference to guide precise antidepressant treatment in different gender population in clinical care. PeerJ Inc. 2022-09-16 /pmc/articles/PMC9484450/ /pubmed/36132219 http://dx.doi.org/10.7717/peerj.14014 Text en ©2022 Chen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Animal Behavior Chen, Yitian Cai, Weijia Li, Canye Su, Zuanjun Guo, Zhijun Li, Zhuman Wang, Chen Xu, Feng Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title | Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title_full | Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title_fullStr | Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title_full_unstemmed | Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title_short | Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
title_sort | sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype |
topic | Animal Behavior |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484450/ https://www.ncbi.nlm.nih.gov/pubmed/36132219 http://dx.doi.org/10.7717/peerj.14014 |
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