Cargando…
Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer
BACKGROUND: Cisplatin resistance is observed in patients with laryngeal cancer. The present study was designed to explore the efficacy of oxaliplatin on laryngeal cancer and elucidate the underlying mechanisms. METHODS: Cell viability was determined by using MTT assays. Cell apoptosis was determined...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484908/ https://www.ncbi.nlm.nih.gov/pubmed/36131787 http://dx.doi.org/10.1155/2022/3760766 |
_version_ | 1784791976346910720 |
---|---|
author | Wang, Jingmiao Zhang, Haizhong Yin, Xiaoyan Bian, Yanrui |
author_facet | Wang, Jingmiao Zhang, Haizhong Yin, Xiaoyan Bian, Yanrui |
author_sort | Wang, Jingmiao |
collection | PubMed |
description | BACKGROUND: Cisplatin resistance is observed in patients with laryngeal cancer. The present study was designed to explore the efficacy of oxaliplatin on laryngeal cancer and elucidate the underlying mechanisms. METHODS: Cell viability was determined by using MTT assays. Cell apoptosis was determined by using annexin V and propidium iodide (PI) staining. Flow cytometry and immunofluorescence were applied to determine the levels of calreticulin (CALR) and DiD (1,1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine). Flow cytometry was applied to analyze the levels of CD83, CD86, IFN-γ-producing CD8(+) T cells, and CD4(+)CD25(+)FoxP3(+) Tregs. The levels of adenosine triphosphate (ATP) were determined by using a chemiluminescent ATP kit and cytokines were determined by using specific enzyme-linked immunosorbent assays (ELISAs). The levels of HMGB1 were determined by using Western blot and ELISA, respectively. The xenograft animal model was constructed to evaluate the antitumor effects of oxaliplatin. RESULTS: Oxaliplatin inhibited cell growth, promoted cell apoptosis, and induced the levels of CALR, ATP, and high mobility group box protein 1 (HMGB1) in Hep-2 cells. Oxaliplatin-treated Hep-2 cells increased the intensity of DiD and the levels of CD83 and CD86 in dendritic cells (DCs), as well as induced the supernatant IL-6 and TNF-α. Oxaliplatin-treated primary laryngeal cancer cell-pulsed DCs increased the IFN-γ-producing CD8(+) T cells and suppressed CD4(+)CD25(+)FoxP3(+) Tregs. In vivo data showed that oxaliplatin suppressed tumor growth and increased the populations of CD86(+)CD80(+) and CD8(+)CD45(+) cells in the tumor tissues. CONCLUSION: Treatment with oxaliplatin inhibited laryngeal cancer cells by inducing immunogenic cell death. |
format | Online Article Text |
id | pubmed-9484908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-94849082022-09-20 Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer Wang, Jingmiao Zhang, Haizhong Yin, Xiaoyan Bian, Yanrui J Oncol Research Article BACKGROUND: Cisplatin resistance is observed in patients with laryngeal cancer. The present study was designed to explore the efficacy of oxaliplatin on laryngeal cancer and elucidate the underlying mechanisms. METHODS: Cell viability was determined by using MTT assays. Cell apoptosis was determined by using annexin V and propidium iodide (PI) staining. Flow cytometry and immunofluorescence were applied to determine the levels of calreticulin (CALR) and DiD (1,1-dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine). Flow cytometry was applied to analyze the levels of CD83, CD86, IFN-γ-producing CD8(+) T cells, and CD4(+)CD25(+)FoxP3(+) Tregs. The levels of adenosine triphosphate (ATP) were determined by using a chemiluminescent ATP kit and cytokines were determined by using specific enzyme-linked immunosorbent assays (ELISAs). The levels of HMGB1 were determined by using Western blot and ELISA, respectively. The xenograft animal model was constructed to evaluate the antitumor effects of oxaliplatin. RESULTS: Oxaliplatin inhibited cell growth, promoted cell apoptosis, and induced the levels of CALR, ATP, and high mobility group box protein 1 (HMGB1) in Hep-2 cells. Oxaliplatin-treated Hep-2 cells increased the intensity of DiD and the levels of CD83 and CD86 in dendritic cells (DCs), as well as induced the supernatant IL-6 and TNF-α. Oxaliplatin-treated primary laryngeal cancer cell-pulsed DCs increased the IFN-γ-producing CD8(+) T cells and suppressed CD4(+)CD25(+)FoxP3(+) Tregs. In vivo data showed that oxaliplatin suppressed tumor growth and increased the populations of CD86(+)CD80(+) and CD8(+)CD45(+) cells in the tumor tissues. CONCLUSION: Treatment with oxaliplatin inhibited laryngeal cancer cells by inducing immunogenic cell death. Hindawi 2022-09-12 /pmc/articles/PMC9484908/ /pubmed/36131787 http://dx.doi.org/10.1155/2022/3760766 Text en Copyright © 2022 Jingmiao Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Jingmiao Zhang, Haizhong Yin, Xiaoyan Bian, Yanrui Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title | Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title_full | Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title_fullStr | Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title_full_unstemmed | Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title_short | Oxaliplatin Induces Immunogenic Cell Death in Human and Murine Laryngeal Cancer |
title_sort | oxaliplatin induces immunogenic cell death in human and murine laryngeal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484908/ https://www.ncbi.nlm.nih.gov/pubmed/36131787 http://dx.doi.org/10.1155/2022/3760766 |
work_keys_str_mv | AT wangjingmiao oxaliplatininducesimmunogeniccelldeathinhumanandmurinelaryngealcancer AT zhanghaizhong oxaliplatininducesimmunogeniccelldeathinhumanandmurinelaryngealcancer AT yinxiaoyan oxaliplatininducesimmunogeniccelldeathinhumanandmurinelaryngealcancer AT bianyanrui oxaliplatininducesimmunogeniccelldeathinhumanandmurinelaryngealcancer |