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Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird
Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour. The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isola...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484970/ https://www.ncbi.nlm.nih.gov/pubmed/36132226 http://dx.doi.org/10.1155/2022/7627759 |
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author | Muneer, Afshan Kumar, Santosh Aqib, Amjad Islam Khan, Shanza Rauf Shah, Syed Qaswar Ali Zaheer, Iqra Rehman, Tauseef ur Abbas, Asghar Hussain, Kashif Rehman, Atif Nadeem, Muhammad Murtaza, Maheen Waseem, Ahmad |
author_facet | Muneer, Afshan Kumar, Santosh Aqib, Amjad Islam Khan, Shanza Rauf Shah, Syed Qaswar Ali Zaheer, Iqra Rehman, Tauseef ur Abbas, Asghar Hussain, Kashif Rehman, Atif Nadeem, Muhammad Murtaza, Maheen Waseem, Ahmad |
author_sort | Muneer, Afshan |
collection | PubMed |
description | Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour. The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples). The preparations consisted of gel stabilized ampicillin (G+A), gel stabilized MgO and ampicillin (G+M+A), gel stabilized MgO and cefoxitin (G+M+C), gel stabilized tylosin (G+T), gel stabilized MgO and tylosin (G+M+T), and gel stabilized MgO (M+G). The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs. G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T. Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16(th) hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24(th) hr of incubation. In the case of G+T and M+G, significant reduction in MIC was found at the 20(th) hr and 24(th) hr of incubation. Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations. The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity. |
format | Online Article Text |
id | pubmed-9484970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-94849702022-09-20 Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird Muneer, Afshan Kumar, Santosh Aqib, Amjad Islam Khan, Shanza Rauf Shah, Syed Qaswar Ali Zaheer, Iqra Rehman, Tauseef ur Abbas, Asghar Hussain, Kashif Rehman, Atif Nadeem, Muhammad Murtaza, Maheen Waseem, Ahmad Oxid Med Cell Longev Research Article Alternative approaches and/or modified approaches to tackle resistance in gut microbes are need of the hour. The current study was planned to find the resistance modulation and toxicity potential of sodium alginate stabilized MgO nanoparticles and antibiotics against Escherichia coli (E. coli) isolated from the gut of Houbara bustard bird (n = 105 fecal samples). The preparations consisted of gel stabilized ampicillin (G+A), gel stabilized MgO and ampicillin (G+M+A), gel stabilized MgO and cefoxitin (G+M+C), gel stabilized tylosin (G+T), gel stabilized MgO and tylosin (G+M+T), and gel stabilized MgO (M+G). The fecal samples showed 53% (56/105) prevalence of E. coli which was found to be significantly (p < 0.05) associated with most of the assumed factors and resistant to multiple drugs. G+M+T showed the lowest (4.883 ± 0.00μg/mL) minimum inhibitory concentration (MIC) followed G+M+C, G+M+A, G+A, M+G, and G+T. Significant reduction (p < 0.05) in MIC with respect to incubation interval found at the 16(th) hr for G+M+A, G+A, and G+M+C that further remained nonsignificant (p > 0.05) onwards until the 24(th) hr of incubation. In the case of G+T and M+G, significant reduction in MIC was found at the 20(th) hr and 24(th) hr of incubation. Ecotoxicology and histopathology trials on snails showed mild changes in MICs of the preparations. The study thus concluded increasing drug resistance in E. coli of houbara bird while sodium alginate stabilized MgO nanoparticles and antibiotics were effective alternative antibacterial composites with mild toxicity. Hindawi 2022-09-12 /pmc/articles/PMC9484970/ /pubmed/36132226 http://dx.doi.org/10.1155/2022/7627759 Text en Copyright © 2022 Afshan Muneer et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Muneer, Afshan Kumar, Santosh Aqib, Amjad Islam Khan, Shanza Rauf Shah, Syed Qaswar Ali Zaheer, Iqra Rehman, Tauseef ur Abbas, Asghar Hussain, Kashif Rehman, Atif Nadeem, Muhammad Murtaza, Maheen Waseem, Ahmad Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title | Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title_full | Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title_fullStr | Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title_full_unstemmed | Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title_short | Evaluation of Sodium Alginate Stabilized Nanoparticles and Antibiotics against Drug Resistant Escherichia coli Isolated from Gut of Houbara Bustard Bird |
title_sort | evaluation of sodium alginate stabilized nanoparticles and antibiotics against drug resistant escherichia coli isolated from gut of houbara bustard bird |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484970/ https://www.ncbi.nlm.nih.gov/pubmed/36132226 http://dx.doi.org/10.1155/2022/7627759 |
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