Detection of m(6)A RNA modifications at single-nucleotide resolution using m(6)A-selective allyl chemical labeling and sequencing

As the most abundant internal mRNA modification, N(6)-methyladenosine (m(6)A) was involved in almost all the aspects of RNA metabolism. Here, we introduce our protocol for m(6)A-SAC-seq, which enables the whole transcriptome-wide mapping of m(6)A RNA modification at single-nucleotide resolution with...

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Detalles Bibliográficos
Autores principales: Peng, Yong, Meng, Hanzhe, Ge, Ruiqi, Liu, Shun, Chen, Mengjie, He, Chuan, Hu, Lulu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485529/
https://www.ncbi.nlm.nih.gov/pubmed/36112507
http://dx.doi.org/10.1016/j.xpro.2022.101677

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