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Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo

Methicillin-resistant Staphylococcus aureus (MRSA) is among the common drug resistant bacteria, which has gained worldwide attention due to its high drug resistance and infection rates. Biofilms produced by S. aureus are known to increase antibiotic resistance, making the treatment of S. aureus infe...

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Autores principales: Gu, Kexin, Ouyang, Ping, Hong, Yuxin, Dai, Yuyun, Tang, Ting, He, Changliang, Shu, Gang, Liang, Xiaoxia, Tang, Huaqiao, Zhu, Ling, Xu, Zhiwen, Yin, Lizi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485828/
https://www.ncbi.nlm.nih.gov/pubmed/36147840
http://dx.doi.org/10.3389/fmicb.2022.960728
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author Gu, Kexin
Ouyang, Ping
Hong, Yuxin
Dai, Yuyun
Tang, Ting
He, Changliang
Shu, Gang
Liang, Xiaoxia
Tang, Huaqiao
Zhu, Ling
Xu, Zhiwen
Yin, Lizi
author_facet Gu, Kexin
Ouyang, Ping
Hong, Yuxin
Dai, Yuyun
Tang, Ting
He, Changliang
Shu, Gang
Liang, Xiaoxia
Tang, Huaqiao
Zhu, Ling
Xu, Zhiwen
Yin, Lizi
author_sort Gu, Kexin
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) is among the common drug resistant bacteria, which has gained worldwide attention due to its high drug resistance and infection rates. Biofilms produced by S. aureus are known to increase antibiotic resistance, making the treatment of S. aureus infections even more challenging. Hence, inhibition of biofilm formation has become an alternative strategy for controlling persistent infections. In this study, we evaluated the efficacy of geraniol as a treatment for MRSA biofilm infection. The results of crystal violet staining indicated that 256 μg/mL concentration of geraniol inhibited USA300 biofilm formation by 86.13% and removed mature biofilms by 49.87%. Geraniol exerted its anti-biofilm effect by influencing the major components of the MRSA biofilm structure. We found that geraniol inhibited the synthesis of major virulence factors, including staphyloxanthin and autolysins. The colony count revealed that geraniol inhibited staphyloxanthin and sensitized USA300 cells to hydrogen peroxide. Interestingly, geraniol not only reduced the release of extracellular nucleic acids (eDNA) but also inhibited cell autolysis. Real-time polymerase chain reaction data revealed the downregulation of genes involved in biofilm formation, which verified the results of the phenotypic analysis. Geraniol increased the effect of vancomycin in eliminating USA300 biofilms in a mouse infection model. Our findings revealed that geraniol effectively inhibits biofilm formation in vitro. Furthermore, in combination with vancomycin, geraniol can reduce the biofilm adhesion to the implant in mice. This suggests the potential of geraniol as an anti-MRSA biofilm drug and can provide a solution for the clinical treatment of biofilm infection.
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spelling pubmed-94858282022-09-21 Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo Gu, Kexin Ouyang, Ping Hong, Yuxin Dai, Yuyun Tang, Ting He, Changliang Shu, Gang Liang, Xiaoxia Tang, Huaqiao Zhu, Ling Xu, Zhiwen Yin, Lizi Front Microbiol Microbiology Methicillin-resistant Staphylococcus aureus (MRSA) is among the common drug resistant bacteria, which has gained worldwide attention due to its high drug resistance and infection rates. Biofilms produced by S. aureus are known to increase antibiotic resistance, making the treatment of S. aureus infections even more challenging. Hence, inhibition of biofilm formation has become an alternative strategy for controlling persistent infections. In this study, we evaluated the efficacy of geraniol as a treatment for MRSA biofilm infection. The results of crystal violet staining indicated that 256 μg/mL concentration of geraniol inhibited USA300 biofilm formation by 86.13% and removed mature biofilms by 49.87%. Geraniol exerted its anti-biofilm effect by influencing the major components of the MRSA biofilm structure. We found that geraniol inhibited the synthesis of major virulence factors, including staphyloxanthin and autolysins. The colony count revealed that geraniol inhibited staphyloxanthin and sensitized USA300 cells to hydrogen peroxide. Interestingly, geraniol not only reduced the release of extracellular nucleic acids (eDNA) but also inhibited cell autolysis. Real-time polymerase chain reaction data revealed the downregulation of genes involved in biofilm formation, which verified the results of the phenotypic analysis. Geraniol increased the effect of vancomycin in eliminating USA300 biofilms in a mouse infection model. Our findings revealed that geraniol effectively inhibits biofilm formation in vitro. Furthermore, in combination with vancomycin, geraniol can reduce the biofilm adhesion to the implant in mice. This suggests the potential of geraniol as an anti-MRSA biofilm drug and can provide a solution for the clinical treatment of biofilm infection. Frontiers Media S.A. 2022-09-06 /pmc/articles/PMC9485828/ /pubmed/36147840 http://dx.doi.org/10.3389/fmicb.2022.960728 Text en Copyright © 2022 Gu, Ouyang, Hong, Dai, Tang, He, Shu, Liang, Tang, Zhu, Xu and Yin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gu, Kexin
Ouyang, Ping
Hong, Yuxin
Dai, Yuyun
Tang, Ting
He, Changliang
Shu, Gang
Liang, Xiaoxia
Tang, Huaqiao
Zhu, Ling
Xu, Zhiwen
Yin, Lizi
Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title_full Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title_fullStr Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title_full_unstemmed Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title_short Geraniol inhibits biofilm formation of methicillin-resistant Staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
title_sort geraniol inhibits biofilm formation of methicillin-resistant staphylococcus aureus and increase the therapeutic effect of vancomycin in vivo
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485828/
https://www.ncbi.nlm.nih.gov/pubmed/36147840
http://dx.doi.org/10.3389/fmicb.2022.960728
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