Lipid profiling analyses from mouse models and human infants

This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimenta...

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Detalles Bibliográficos
Autores principales: Olga, Laurentya, Bobeldijk-Pastorova, Ivana, Bas, Richard C., Seidel, Florine, Snowden, Stuart G., Furse, Samuel, Ong, Ken K., Kleemann, Robert, Koulman, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486117/
https://www.ncbi.nlm.nih.gov/pubmed/36115026
http://dx.doi.org/10.1016/j.xpro.2022.101679
Descripción
Sumario:This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort. For complete details on the use and execution of this protocol, please refer to Olga et al. (2021).

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