Overcoming Leak Sensitivity in CRISPRi Circuits Using Antisense RNA Sequestration and Regulatory Feedback

[Image: see text] The controlled binding of the catalytically dead CRISPR nuclease (dCas) to DNA can be used to create complex, programmable transcriptional genetic circuits, a fundamental goal of synthetic biology. This approach, called CRISPR interference (CRISPRi), is advantageous over existing m...

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Detalles Bibliográficos
Autores principales: Specht, David A., Cortes, Louis B., Lambert, Guillaume
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486968/
https://www.ncbi.nlm.nih.gov/pubmed/36017994
http://dx.doi.org/10.1021/acssynbio.2c00155

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486968/
https://www.ncbi.nlm.nih.gov/pubmed/36017994
http://dx.doi.org/10.1021/acssynbio.2c00155

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