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A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro

OBJECTIVES: To test the effect of probiotic strains (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12), a prebiotic (bacteriophage blend), and a postbiotic (tributyrin) on the human intestinal microbiota in vitro. METHODS: Short term colonic incubation was performed under...

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Autores principales: Vance, Arianne, Raut, Shilpa, Alamdari, Nima, Grier-Welch, Adeline, Sharafi, Mastaneh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9487199/
http://dx.doi.org/10.1093/cdn/nzac069.038
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author Vance, Arianne
Raut, Shilpa
Alamdari, Nima
Grier-Welch, Adeline
Sharafi, Mastaneh
author_facet Vance, Arianne
Raut, Shilpa
Alamdari, Nima
Grier-Welch, Adeline
Sharafi, Mastaneh
author_sort Vance, Arianne
collection PubMed
description OBJECTIVES: To test the effect of probiotic strains (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12), a prebiotic (bacteriophage blend), and a postbiotic (tributyrin) on the human intestinal microbiota in vitro. METHODS: Short term colonic incubation was performed under conditions representative of the proximal large intestine, using the fecal microbiota of a single healthy human donor. At the start of the experiment, treatments and a blank control were added to the reactors. Each reactor also received a carbohydrate-supplemented nutritional medium and freshly prepared fecal inoculum. Reactors were closed with a rubber septum, made anaerobic through flushing with nitrogen, and incubated for 48 hours. Each condition was run in triplicate. Changes in microbial community composition were determined via 16S-targeted Illumina sequencing and flow cytometry. Enriched bacterial genera were identified using LEfSe (LDA > 2, p < 0.05). RESULTS: A significant increase in alpha-diversity was observed by 48h compared to the control. LEfSe revealed that community shifts were attributed to a wide range of bacterial genera including Gemmiger, Lactobacillus, Bacteroides, Eubacterium, Oscillibacter, and Holdemanella at 24h and of genera such as Lactobacillus, Bacteroides, Muribaculum, and Holdemanella at 48h. LGG and BB-12 were not detected in the donor microbiota originally but abundance of both was higher after incubation. CONCLUSIONS: The results from this study suggest that a combination of pre-, pro-, and postbiotics modulates gut microbiota by increasing microbial diversity and the relative abundance of a number of bacterial genera. An increase in the abundance of LGG and BB-12 indicates growth of these strains in the presence of a human gut microbiota. Future human clinical studies are needed to further investigate the observed effects. FUNDING SOURCES: The study was conducted by an independent lab, ProDigest, and funded and sponsored by Ritual (Natals, Inc.). Compound Solutions, Inc. also financially supported the study.
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spelling pubmed-94871992022-09-21 A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro Vance, Arianne Raut, Shilpa Alamdari, Nima Grier-Welch, Adeline Sharafi, Mastaneh Curr Dev Nutr Nutritional Microbiology/Microbiome OBJECTIVES: To test the effect of probiotic strains (Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB-12), a prebiotic (bacteriophage blend), and a postbiotic (tributyrin) on the human intestinal microbiota in vitro. METHODS: Short term colonic incubation was performed under conditions representative of the proximal large intestine, using the fecal microbiota of a single healthy human donor. At the start of the experiment, treatments and a blank control were added to the reactors. Each reactor also received a carbohydrate-supplemented nutritional medium and freshly prepared fecal inoculum. Reactors were closed with a rubber septum, made anaerobic through flushing with nitrogen, and incubated for 48 hours. Each condition was run in triplicate. Changes in microbial community composition were determined via 16S-targeted Illumina sequencing and flow cytometry. Enriched bacterial genera were identified using LEfSe (LDA > 2, p < 0.05). RESULTS: A significant increase in alpha-diversity was observed by 48h compared to the control. LEfSe revealed that community shifts were attributed to a wide range of bacterial genera including Gemmiger, Lactobacillus, Bacteroides, Eubacterium, Oscillibacter, and Holdemanella at 24h and of genera such as Lactobacillus, Bacteroides, Muribaculum, and Holdemanella at 48h. LGG and BB-12 were not detected in the donor microbiota originally but abundance of both was higher after incubation. CONCLUSIONS: The results from this study suggest that a combination of pre-, pro-, and postbiotics modulates gut microbiota by increasing microbial diversity and the relative abundance of a number of bacterial genera. An increase in the abundance of LGG and BB-12 indicates growth of these strains in the presence of a human gut microbiota. Future human clinical studies are needed to further investigate the observed effects. FUNDING SOURCES: The study was conducted by an independent lab, ProDigest, and funded and sponsored by Ritual (Natals, Inc.). Compound Solutions, Inc. also financially supported the study. Oxford University Press 2022-06-14 /pmc/articles/PMC9487199/ http://dx.doi.org/10.1093/cdn/nzac069.038 Text en © The Author 2022. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Nutritional Microbiology/Microbiome
Vance, Arianne
Raut, Shilpa
Alamdari, Nima
Grier-Welch, Adeline
Sharafi, Mastaneh
A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title_full A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title_fullStr A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title_full_unstemmed A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title_short A Novel Combination of Pre-, Pro-, and Postbiotics Modulates Bacterial Diversity and Composition In Vitro
title_sort novel combination of pre-, pro-, and postbiotics modulates bacterial diversity and composition in vitro
topic Nutritional Microbiology/Microbiome
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9487199/
http://dx.doi.org/10.1093/cdn/nzac069.038
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