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The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were fu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488000/ https://www.ncbi.nlm.nih.gov/pubmed/36147853 http://dx.doi.org/10.3389/fmicb.2022.962500 |
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author | Li, Ling Yu, Meng-xue Guo, Jian Hao, Zhong-na Zhang, Zhen Lu, Zi-qi Wang, Jiao-yu Zhu, Xue-ming Wang, Yan-li Chen, Jie Sun, Guo-Chang Lin, Fu-cheng |
author_facet | Li, Ling Yu, Meng-xue Guo, Jian Hao, Zhong-na Zhang, Zhen Lu, Zi-qi Wang, Jiao-yu Zhu, Xue-ming Wang, Yan-li Chen, Jie Sun, Guo-Chang Lin, Fu-cheng |
author_sort | Li, Ling |
collection | PubMed |
description | Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were functionally characterized in this work using gene disruption strategies. Compared with the wild-type strain (WT), the Δbcpex8, Δbcpex10, and Δbcpex12 mutants exhibited significant reduction in melanin production, fatty acid utilization, and decreased tolerance to high osmotic pressure and reactive oxygen species (ROS). The mycelial growth and conidiation of were significantly inhibited in Δbcpex8, Δbcpex10, and Δbcpex12 strains. The mycelial growth rates of Δbcpex8, Δbcpex10, and Δbcpex12 were reduced by 32, 35, and 34%, respectively, compared with WT and ectopic transformant (ET), and the conidiation was reduced by approximately 89, 27, and 88%, respectively. The conidial germination, germ tube elongation, and the formation of initiate infection structures (IFSs) were also reduced by the deletion of the genes. The pathogenicity was tested on the leaves of tobacco and strawberry, and fruits of tomato. On the leaves of tobacco and strawberry, the Δbcpex8, Δbcpex10, and Δbcpex12 mutants could not induce necrotic lesions, and the lesions on tomato fruits infected with the mutants were significantly reduced than those of the wide type. The results indicated that BcPEX8, BcPEX10, and BcPEX12 are indispensable for the development and pathogenicity of B. cinerea. |
format | Online Article Text |
id | pubmed-9488000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94880002022-09-21 The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea Li, Ling Yu, Meng-xue Guo, Jian Hao, Zhong-na Zhang, Zhen Lu, Zi-qi Wang, Jiao-yu Zhu, Xue-ming Wang, Yan-li Chen, Jie Sun, Guo-Chang Lin, Fu-cheng Front Microbiol Microbiology Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were functionally characterized in this work using gene disruption strategies. Compared with the wild-type strain (WT), the Δbcpex8, Δbcpex10, and Δbcpex12 mutants exhibited significant reduction in melanin production, fatty acid utilization, and decreased tolerance to high osmotic pressure and reactive oxygen species (ROS). The mycelial growth and conidiation of were significantly inhibited in Δbcpex8, Δbcpex10, and Δbcpex12 strains. The mycelial growth rates of Δbcpex8, Δbcpex10, and Δbcpex12 were reduced by 32, 35, and 34%, respectively, compared with WT and ectopic transformant (ET), and the conidiation was reduced by approximately 89, 27, and 88%, respectively. The conidial germination, germ tube elongation, and the formation of initiate infection structures (IFSs) were also reduced by the deletion of the genes. The pathogenicity was tested on the leaves of tobacco and strawberry, and fruits of tomato. On the leaves of tobacco and strawberry, the Δbcpex8, Δbcpex10, and Δbcpex12 mutants could not induce necrotic lesions, and the lesions on tomato fruits infected with the mutants were significantly reduced than those of the wide type. The results indicated that BcPEX8, BcPEX10, and BcPEX12 are indispensable for the development and pathogenicity of B. cinerea. Frontiers Media S.A. 2022-09-06 /pmc/articles/PMC9488000/ /pubmed/36147853 http://dx.doi.org/10.3389/fmicb.2022.962500 Text en Copyright © 2022 Li, Yu, Guo, Hao, Zhang, Lu, Wang, Zhu, Wang, Chen, Sun and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Ling Yu, Meng-xue Guo, Jian Hao, Zhong-na Zhang, Zhen Lu, Zi-qi Wang, Jiao-yu Zhu, Xue-ming Wang, Yan-li Chen, Jie Sun, Guo-Chang Lin, Fu-cheng The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title | The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title_full | The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title_fullStr | The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title_full_unstemmed | The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title_short | The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea |
title_sort | peroxins bcpex8, bcpex10, and bcpex12 are required for the development and pathogenicity of botrytis cinerea |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488000/ https://www.ncbi.nlm.nih.gov/pubmed/36147853 http://dx.doi.org/10.3389/fmicb.2022.962500 |
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