Cargando…

The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea

Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were fu...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ling, Yu, Meng-xue, Guo, Jian, Hao, Zhong-na, Zhang, Zhen, Lu, Zi-qi, Wang, Jiao-yu, Zhu, Xue-ming, Wang, Yan-li, Chen, Jie, Sun, Guo-Chang, Lin, Fu-cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488000/
https://www.ncbi.nlm.nih.gov/pubmed/36147853
http://dx.doi.org/10.3389/fmicb.2022.962500
_version_ 1784792562979045376
author Li, Ling
Yu, Meng-xue
Guo, Jian
Hao, Zhong-na
Zhang, Zhen
Lu, Zi-qi
Wang, Jiao-yu
Zhu, Xue-ming
Wang, Yan-li
Chen, Jie
Sun, Guo-Chang
Lin, Fu-cheng
author_facet Li, Ling
Yu, Meng-xue
Guo, Jian
Hao, Zhong-na
Zhang, Zhen
Lu, Zi-qi
Wang, Jiao-yu
Zhu, Xue-ming
Wang, Yan-li
Chen, Jie
Sun, Guo-Chang
Lin, Fu-cheng
author_sort Li, Ling
collection PubMed
description Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were functionally characterized in this work using gene disruption strategies. Compared with the wild-type strain (WT), the Δbcpex8, Δbcpex10, and Δbcpex12 mutants exhibited significant reduction in melanin production, fatty acid utilization, and decreased tolerance to high osmotic pressure and reactive oxygen species (ROS). The mycelial growth and conidiation of were significantly inhibited in Δbcpex8, Δbcpex10, and Δbcpex12 strains. The mycelial growth rates of Δbcpex8, Δbcpex10, and Δbcpex12 were reduced by 32, 35, and 34%, respectively, compared with WT and ectopic transformant (ET), and the conidiation was reduced by approximately 89, 27, and 88%, respectively. The conidial germination, germ tube elongation, and the formation of initiate infection structures (IFSs) were also reduced by the deletion of the genes. The pathogenicity was tested on the leaves of tobacco and strawberry, and fruits of tomato. On the leaves of tobacco and strawberry, the Δbcpex8, Δbcpex10, and Δbcpex12 mutants could not induce necrotic lesions, and the lesions on tomato fruits infected with the mutants were significantly reduced than those of the wide type. The results indicated that BcPEX8, BcPEX10, and BcPEX12 are indispensable for the development and pathogenicity of B. cinerea.
format Online
Article
Text
id pubmed-9488000
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-94880002022-09-21 The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea Li, Ling Yu, Meng-xue Guo, Jian Hao, Zhong-na Zhang, Zhen Lu, Zi-qi Wang, Jiao-yu Zhu, Xue-ming Wang, Yan-li Chen, Jie Sun, Guo-Chang Lin, Fu-cheng Front Microbiol Microbiology Peroxisomes have been proved playing roles in infection of several plant pathogens. Although the contribution of a portion of peroxins in pathogenicity was demonstrated, most of them are undocumented in fungi, especially, Botrytis cinerea. The homologs of Pex8, Pex10, and Pex12 in B. cinerea were functionally characterized in this work using gene disruption strategies. Compared with the wild-type strain (WT), the Δbcpex8, Δbcpex10, and Δbcpex12 mutants exhibited significant reduction in melanin production, fatty acid utilization, and decreased tolerance to high osmotic pressure and reactive oxygen species (ROS). The mycelial growth and conidiation of were significantly inhibited in Δbcpex8, Δbcpex10, and Δbcpex12 strains. The mycelial growth rates of Δbcpex8, Δbcpex10, and Δbcpex12 were reduced by 32, 35, and 34%, respectively, compared with WT and ectopic transformant (ET), and the conidiation was reduced by approximately 89, 27, and 88%, respectively. The conidial germination, germ tube elongation, and the formation of initiate infection structures (IFSs) were also reduced by the deletion of the genes. The pathogenicity was tested on the leaves of tobacco and strawberry, and fruits of tomato. On the leaves of tobacco and strawberry, the Δbcpex8, Δbcpex10, and Δbcpex12 mutants could not induce necrotic lesions, and the lesions on tomato fruits infected with the mutants were significantly reduced than those of the wide type. The results indicated that BcPEX8, BcPEX10, and BcPEX12 are indispensable for the development and pathogenicity of B. cinerea. Frontiers Media S.A. 2022-09-06 /pmc/articles/PMC9488000/ /pubmed/36147853 http://dx.doi.org/10.3389/fmicb.2022.962500 Text en Copyright © 2022 Li, Yu, Guo, Hao, Zhang, Lu, Wang, Zhu, Wang, Chen, Sun and Lin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Ling
Yu, Meng-xue
Guo, Jian
Hao, Zhong-na
Zhang, Zhen
Lu, Zi-qi
Wang, Jiao-yu
Zhu, Xue-ming
Wang, Yan-li
Chen, Jie
Sun, Guo-Chang
Lin, Fu-cheng
The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title_full The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title_fullStr The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title_full_unstemmed The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title_short The peroxins BcPex8, BcPex10, and BcPex12 are required for the development and pathogenicity of Botrytis cinerea
title_sort peroxins bcpex8, bcpex10, and bcpex12 are required for the development and pathogenicity of botrytis cinerea
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488000/
https://www.ncbi.nlm.nih.gov/pubmed/36147853
http://dx.doi.org/10.3389/fmicb.2022.962500
work_keys_str_mv AT liling theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT yumengxue theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT guojian theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT haozhongna theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT zhangzhen theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT luziqi theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT wangjiaoyu theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT zhuxueming theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT wangyanli theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT chenjie theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT sunguochang theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT linfucheng theperoxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT liling peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT yumengxue peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT guojian peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT haozhongna peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT zhangzhen peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT luziqi peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT wangjiaoyu peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT zhuxueming peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT wangyanli peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT chenjie peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT sunguochang peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea
AT linfucheng peroxinsbcpex8bcpex10andbcpex12arerequiredforthedevelopmentandpathogenicityofbotrytiscinerea