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Acellular human lung scaffolds to model lung disease and tissue regeneration
Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed us...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Respiratory Society
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488127/ https://www.ncbi.nlm.nih.gov/pubmed/29875137 http://dx.doi.org/10.1183/16000617.0021-2018 |
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author | Gilpin, Sarah E. Wagner, Darcy E. |
author_facet | Gilpin, Sarah E. Wagner, Darcy E. |
author_sort | Gilpin, Sarah E. |
collection | PubMed |
description | Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed using both perfusion and immersion based techniques. Immersion based techniques allow laboratories without access to intact lobes the ability to generate acellular human lung scaffolds. Acellular human lung scaffolds can be further processed into small segments, thin slices or extracellular matrix extracts, to study cell behaviour such as viability, proliferation, migration and differentiation. Recent studies have offered important proof of concept of generating sufficient primary endothelial and lung epithelial cells to recellularise whole lobes that can be maintained for several days ex vivo in a bioreactor to study regeneration. In parallel, acellular human lung scaffolds have been increasingly used for studying cell–extracellular environment interactions. These studies have helped provide new insights into the role of the matrix and the extracellular environment in chronic human lung diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. Acellular human lung scaffolds are a versatile new tool for studying human lung repair and regeneration ex vivo. |
format | Online Article Text |
id | pubmed-9488127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | European Respiratory Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-94881272022-11-14 Acellular human lung scaffolds to model lung disease and tissue regeneration Gilpin, Sarah E. Wagner, Darcy E. Eur Respir Rev Lung Science Conference Recent advances in whole lung bioengineering have opened new doors for studying lung repair and regeneration ex vivo using acellular human derived lung tissue scaffolds. Methods to decellularise whole human lungs, lobes or resected segments from normal and diseased human lungs have been developed using both perfusion and immersion based techniques. Immersion based techniques allow laboratories without access to intact lobes the ability to generate acellular human lung scaffolds. Acellular human lung scaffolds can be further processed into small segments, thin slices or extracellular matrix extracts, to study cell behaviour such as viability, proliferation, migration and differentiation. Recent studies have offered important proof of concept of generating sufficient primary endothelial and lung epithelial cells to recellularise whole lobes that can be maintained for several days ex vivo in a bioreactor to study regeneration. In parallel, acellular human lung scaffolds have been increasingly used for studying cell–extracellular environment interactions. These studies have helped provide new insights into the role of the matrix and the extracellular environment in chronic human lung diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. Acellular human lung scaffolds are a versatile new tool for studying human lung repair and regeneration ex vivo. European Respiratory Society 2018-06-06 /pmc/articles/PMC9488127/ /pubmed/29875137 http://dx.doi.org/10.1183/16000617.0021-2018 Text en Copyright ©ERS 2018. https://creativecommons.org/licenses/by-nc/4.0/ERR articles are open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0. |
spellingShingle | Lung Science Conference Gilpin, Sarah E. Wagner, Darcy E. Acellular human lung scaffolds to model lung disease and tissue regeneration |
title | Acellular human lung scaffolds to model lung disease and tissue regeneration |
title_full | Acellular human lung scaffolds to model lung disease and tissue regeneration |
title_fullStr | Acellular human lung scaffolds to model lung disease and tissue regeneration |
title_full_unstemmed | Acellular human lung scaffolds to model lung disease and tissue regeneration |
title_short | Acellular human lung scaffolds to model lung disease and tissue regeneration |
title_sort | acellular human lung scaffolds to model lung disease and tissue regeneration |
topic | Lung Science Conference |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488127/ https://www.ncbi.nlm.nih.gov/pubmed/29875137 http://dx.doi.org/10.1183/16000617.0021-2018 |
work_keys_str_mv | AT gilpinsarahe acellularhumanlungscaffoldstomodellungdiseaseandtissueregeneration AT wagnerdarcye acellularhumanlungscaffoldstomodellungdiseaseandtissueregeneration |