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Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas

Diffuse large B-cell lymphomas (DLBCLs) are the most common lymphoproliferative diseases in dogs. DLBCL diagnosis to date has relied on histopathological analysis; however liquid biopsies have gained attention in recent years as a source of diagnostic and prognostic information. Liquid biopsies can...

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Detalles Bibliográficos
Autores principales: Kulka, Marek, Brennan, Kieran, Mc Gee, Margaret
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488776/
https://www.ncbi.nlm.nih.gov/pubmed/36125986
http://dx.doi.org/10.1371/journal.pone.0274261
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author Kulka, Marek
Brennan, Kieran
Mc Gee, Margaret
author_facet Kulka, Marek
Brennan, Kieran
Mc Gee, Margaret
author_sort Kulka, Marek
collection PubMed
description Diffuse large B-cell lymphomas (DLBCLs) are the most common lymphoproliferative diseases in dogs. DLBCL diagnosis to date has relied on histopathological analysis; however liquid biopsies have gained attention in recent years as a source of diagnostic and prognostic information. Liquid biopsies can be a source of circulating DNA, miRNA, circulating tumour cells or extracellular vesicles (EVs). In this study EVs were isolated from the plasma of healthy dogs, and dogs with lymphoma, and adenocarcinoma by iodixanol density gradient centrifugation. These EVs were positive for the EV markers CD63 and TSG101 and the pan-B cell markers CD79a, CD21, CD45, CD20. NTA analysis revealed that the DLBCL and adenocarcinoma dogs had elevated plasma EVs relative to the healthy dogs. Furthermore, the modal size of lymphoma EVs had decreased relative to healthy dogs while adenocarcinoma EVs were unchanged. This study demonstrates that the plasma EV population is altered in canine lymphoma patients in a manner similar to previous studies on human lymphomas. The similar changes to the EV population in dogs, together with the similar pathological features and treatment protocols in canine and human non-Hodgkin lymphomas would make dogs a good comparative model for studying the role of EVs in DLBCL development and progression.
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spelling pubmed-94887762022-09-21 Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas Kulka, Marek Brennan, Kieran Mc Gee, Margaret PLoS One Research Article Diffuse large B-cell lymphomas (DLBCLs) are the most common lymphoproliferative diseases in dogs. DLBCL diagnosis to date has relied on histopathological analysis; however liquid biopsies have gained attention in recent years as a source of diagnostic and prognostic information. Liquid biopsies can be a source of circulating DNA, miRNA, circulating tumour cells or extracellular vesicles (EVs). In this study EVs were isolated from the plasma of healthy dogs, and dogs with lymphoma, and adenocarcinoma by iodixanol density gradient centrifugation. These EVs were positive for the EV markers CD63 and TSG101 and the pan-B cell markers CD79a, CD21, CD45, CD20. NTA analysis revealed that the DLBCL and adenocarcinoma dogs had elevated plasma EVs relative to the healthy dogs. Furthermore, the modal size of lymphoma EVs had decreased relative to healthy dogs while adenocarcinoma EVs were unchanged. This study demonstrates that the plasma EV population is altered in canine lymphoma patients in a manner similar to previous studies on human lymphomas. The similar changes to the EV population in dogs, together with the similar pathological features and treatment protocols in canine and human non-Hodgkin lymphomas would make dogs a good comparative model for studying the role of EVs in DLBCL development and progression. Public Library of Science 2022-09-20 /pmc/articles/PMC9488776/ /pubmed/36125986 http://dx.doi.org/10.1371/journal.pone.0274261 Text en © 2022 Kulka et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kulka, Marek
Brennan, Kieran
Mc Gee, Margaret
Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title_full Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title_fullStr Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title_full_unstemmed Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title_short Investigation of canine extracellular vesicles in diffuse large B-cell lymphomas
title_sort investigation of canine extracellular vesicles in diffuse large b-cell lymphomas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9488776/
https://www.ncbi.nlm.nih.gov/pubmed/36125986
http://dx.doi.org/10.1371/journal.pone.0274261
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