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Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489141/ https://www.ncbi.nlm.nih.gov/pubmed/36147228 http://dx.doi.org/10.3389/fpls.2022.1005627 |
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author | Mu, Yanjun Gong, Wenping Qie, Yanmin Liu, Xueqing Li, Linzhi Sun, Nina Liu, Wei Guo, Jun Han, Ran Yu, Ziyang Xiao, Luning Su, Fuyu Zhang, Wenjing Wang, Jiangchun Han, Guohao Ma, Pengtao |
author_facet | Mu, Yanjun Gong, Wenping Qie, Yanmin Liu, Xueqing Li, Linzhi Sun, Nina Liu, Wei Guo, Jun Han, Ran Yu, Ziyang Xiao, Luning Su, Fuyu Zhang, Wenjing Wang, Jiangchun Han, Guohao Ma, Pengtao |
author_sort | Mu, Yanjun |
collection | PubMed |
description | Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. |
format | Online Article Text |
id | pubmed-9489141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94891412022-09-21 Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing Mu, Yanjun Gong, Wenping Qie, Yanmin Liu, Xueqing Li, Linzhi Sun, Nina Liu, Wei Guo, Jun Han, Ran Yu, Ziyang Xiao, Luning Su, Fuyu Zhang, Wenjing Wang, Jiangchun Han, Guohao Ma, Pengtao Front Plant Sci Plant Science Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. Frontiers Media S.A. 2022-09-06 /pmc/articles/PMC9489141/ /pubmed/36147228 http://dx.doi.org/10.3389/fpls.2022.1005627 Text en Copyright © 2022 Mu, Gong, Qie, Liu, Li, Sun, Liu, Guo, Han, Yu, Xiao, Su, Zhang, Wang, Han and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Mu, Yanjun Gong, Wenping Qie, Yanmin Liu, Xueqing Li, Linzhi Sun, Nina Liu, Wei Guo, Jun Han, Ran Yu, Ziyang Xiao, Luning Su, Fuyu Zhang, Wenjing Wang, Jiangchun Han, Guohao Ma, Pengtao Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title | Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title_full | Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title_fullStr | Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title_full_unstemmed | Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title_short | Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing |
title_sort | identification of the powdery mildew resistance gene in wheat breeding line yannong 99102-06188 via bulked segregant exome capture sequencing |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489141/ https://www.ncbi.nlm.nih.gov/pubmed/36147228 http://dx.doi.org/10.3389/fpls.2022.1005627 |
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