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Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing

Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and...

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Autores principales: Mu, Yanjun, Gong, Wenping, Qie, Yanmin, Liu, Xueqing, Li, Linzhi, Sun, Nina, Liu, Wei, Guo, Jun, Han, Ran, Yu, Ziyang, Xiao, Luning, Su, Fuyu, Zhang, Wenjing, Wang, Jiangchun, Han, Guohao, Ma, Pengtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489141/
https://www.ncbi.nlm.nih.gov/pubmed/36147228
http://dx.doi.org/10.3389/fpls.2022.1005627
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author Mu, Yanjun
Gong, Wenping
Qie, Yanmin
Liu, Xueqing
Li, Linzhi
Sun, Nina
Liu, Wei
Guo, Jun
Han, Ran
Yu, Ziyang
Xiao, Luning
Su, Fuyu
Zhang, Wenjing
Wang, Jiangchun
Han, Guohao
Ma, Pengtao
author_facet Mu, Yanjun
Gong, Wenping
Qie, Yanmin
Liu, Xueqing
Li, Linzhi
Sun, Nina
Liu, Wei
Guo, Jun
Han, Ran
Yu, Ziyang
Xiao, Luning
Su, Fuyu
Zhang, Wenjing
Wang, Jiangchun
Han, Guohao
Ma, Pengtao
author_sort Mu, Yanjun
collection PubMed
description Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars.
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spelling pubmed-94891412022-09-21 Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing Mu, Yanjun Gong, Wenping Qie, Yanmin Liu, Xueqing Li, Linzhi Sun, Nina Liu, Wei Guo, Jun Han, Ran Yu, Ziyang Xiao, Luning Su, Fuyu Zhang, Wenjing Wang, Jiangchun Han, Guohao Ma, Pengtao Front Plant Sci Plant Science Powdery mildew of wheat (Triticum aestivum), caused by Blumeria graminis f.sp. tritici (Bgt), is a destructive disease that seriously threatens the yield and quality of its host. Identifying resistance genes is the most attractive and effective strategy for developing disease-resistant cultivars and controlling this disease. In this study, a wheat breeding line Yannong 99102-06188 (YN99102), an elite derivative line from the same breeding process as the famous wheat cultivar Yannong 999, showed high resistance to powdery mildew at the whole growth stages. Genetic analysis was carried out using Bgt isolate E09 and a population of YN99102 crossed with a susceptible parent Jinhe 13–205 (JH13–205). The result indicated that a single recessive gene, tentatively designated pmYN99102, conferred seedling resistance to the Bgt isolate E09. Using bulked segregant exome capture sequencing (BSE-Seq), pmYN99102 was physically located to a ~33.7 Mb (691.0–724.7 Mb) interval on the chromosome arm 2BL, and this interval was further locked in a 1.5 cM genetic interval using molecular markers, which was aligned to a 9.0 Mb physical interval (699.2–708.2 Mb). Based on the analysis of physical location, origin, resistant spectrum, and inherited pattern, pmYN99102 differed from those of the reported powdery mildew (Pm) resistance genes on 2BL, suggesting pmYN99102 is most likely a new Pm gene/allele in the targeted interval. To transfer pmYN99102 to different genetic backgrounds using marker-assisted selection (MAS), 18 closely linked markers were tested for their availability in different genetic backgrounds for MAS, and all markers expect for YTU103-97 can be used in MAS for tracking pmYN99102 when it transferred into those susceptible cultivars. Frontiers Media S.A. 2022-09-06 /pmc/articles/PMC9489141/ /pubmed/36147228 http://dx.doi.org/10.3389/fpls.2022.1005627 Text en Copyright © 2022 Mu, Gong, Qie, Liu, Li, Sun, Liu, Guo, Han, Yu, Xiao, Su, Zhang, Wang, Han and Ma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mu, Yanjun
Gong, Wenping
Qie, Yanmin
Liu, Xueqing
Li, Linzhi
Sun, Nina
Liu, Wei
Guo, Jun
Han, Ran
Yu, Ziyang
Xiao, Luning
Su, Fuyu
Zhang, Wenjing
Wang, Jiangchun
Han, Guohao
Ma, Pengtao
Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title_full Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title_fullStr Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title_full_unstemmed Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title_short Identification of the powdery mildew resistance gene in wheat breeding line Yannong 99102-06188 via bulked segregant exome capture sequencing
title_sort identification of the powdery mildew resistance gene in wheat breeding line yannong 99102-06188 via bulked segregant exome capture sequencing
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489141/
https://www.ncbi.nlm.nih.gov/pubmed/36147228
http://dx.doi.org/10.3389/fpls.2022.1005627
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