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Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases

BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of interleukin-15 (IL-15), interleukin-17...

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Autores principales: Khalkhal, Ensieh, Rezaei-Tavirani, Mostafa, Asri, Nastaran, Nobakht, Fatemeh, Jahani-Sherafat, Somayeh, Haidari, Mohammad Hosain, Rostami-Nejad, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Association of Gastroerterology and Hepatology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489306/
https://www.ncbi.nlm.nih.gov/pubmed/36619141
http://dx.doi.org/10.34172/mejdd.2022.272
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author Khalkhal, Ensieh
Rezaei-Tavirani, Mostafa
Asri, Nastaran
Nobakht, Fatemeh
Jahani-Sherafat, Somayeh
Haidari, Mohammad Hosain
Rostami-Nejad, Mohammad
author_facet Khalkhal, Ensieh
Rezaei-Tavirani, Mostafa
Asri, Nastaran
Nobakht, Fatemeh
Jahani-Sherafat, Somayeh
Haidari, Mohammad Hosain
Rostami-Nejad, Mohammad
author_sort Khalkhal, Ensieh
collection PubMed
description BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of interleukin-15 (IL-15), interleukin-17A (IL-17A), interleukin23A (IL-23A), granzyme B (GzmB), T-box transcription factor 21 (TBX21), and tumor necrosis factor alpha-induced protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group. METHODS: Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic (ROC) curve analysis was performed to characterize the diagnostic ability of the studied genes. RESULTS: The expression of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GzmB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls. CONCLUSION: Our results suggest that TNFAIP3, IL23A, and GzmB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated.
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spelling pubmed-94893062023-01-06 Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases Khalkhal, Ensieh Rezaei-Tavirani, Mostafa Asri, Nastaran Nobakht, Fatemeh Jahani-Sherafat, Somayeh Haidari, Mohammad Hosain Rostami-Nejad, Mohammad Middle East J Dig Dis Original Article BACKGROUND: Inflammatory cytokines play roles in the pathogenesis of celiac disease. To introduce new diagnostic markers in patients with celiac disease for easy, fast, low cost, and non-invasive diagnosis, we evaluated the peripheral blood expression levels of interleukin-15 (IL-15), interleukin-17A (IL-17A), interleukin23A (IL-23A), granzyme B (GzmB), T-box transcription factor 21 (TBX21), and tumor necrosis factor alpha-induced protein 3 (TNFAIP3) of patients compared with the healthy controls, which were extracted from public databases organized in a protein-protein interaction network, in this group. METHODS: Peripheral blood mononuclear cells were collected from 30 patients with celiac disease and 30 healthy subjects. Total RNA was extracted, and mRNA expression levels of targeted genes were investigated by the quantitative real-time polymerase chain reaction (PCR) method. SPSS software was used for statistical analysis. Receiver operating characteristic (ROC) curve analysis was performed to characterize the diagnostic ability of the studied genes. RESULTS: The expression of IL-15, IL-17A, IL-23A, GzmB, TBX21, and TNFAIP3 genes in peripheral blood mononuclear cells of patients with celiac disease showed a significant increase compared with the control group. Among them, TNFAIP3, IL23A, and GzmB have better resolution and diagnostic value in differentiating patients with celiac disease from healthy controls. CONCLUSION: Our results suggest that TNFAIP3, IL23A, and GzmB could be useful and sensible markers in differentiating patients with celiac disease from healthy controls. However, the diagnostic relevance of other genes recognized by pathway analysis needs to be further investigated. Iranian Association of Gastroerterology and Hepatology 2022-04 2022-04-30 /pmc/articles/PMC9489306/ /pubmed/36619141 http://dx.doi.org/10.34172/mejdd.2022.272 Text en © 2022 Middle East Journal of Digestive Diseases https://creativecommons.org/licenses/by-nc/4.0/This work is published by Middle East Journal of Digestive Diseases as an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Khalkhal, Ensieh
Rezaei-Tavirani, Mostafa
Asri, Nastaran
Nobakht, Fatemeh
Jahani-Sherafat, Somayeh
Haidari, Mohammad Hosain
Rostami-Nejad, Mohammad
Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title_full Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title_fullStr Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title_full_unstemmed Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title_short Introducing New Potential Biomarkers for Celiac Disease among the Genes Extracted from General Databases
title_sort introducing new potential biomarkers for celiac disease among the genes extracted from general databases
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489306/
https://www.ncbi.nlm.nih.gov/pubmed/36619141
http://dx.doi.org/10.34172/mejdd.2022.272
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