Endogenous protein interactomes resolved through immunoprecipitation-coupled quantitative proteomics in cell lines

Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for the un...

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Detalles Bibliográficos
Autores principales: Kumar, Raman, Kamath, Karthik S., Carroll, Luke, Hoffmann, Peter, Gecz, Jozef, Jolly, Lachlan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489516/
https://www.ncbi.nlm.nih.gov/pubmed/36121748
http://dx.doi.org/10.1016/j.xpro.2022.101693

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