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Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage
Hematopoietic stem cells express differentiation markers B220 and Gr1 and are proliferative. We have shown that the expression of these entities changes with cell cycle passage. Overall, we conclude that primitive hematopoietic stem cells alter their differentiation potential with cell cycle progres...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489557/ https://www.ncbi.nlm.nih.gov/pubmed/35503199 http://dx.doi.org/10.1007/s12015-022-10374-4 |
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author | Goldberg, Laura R. Dooner, Mark S. Papa, Elaine Pereira, Mandy Del Tatto, Michael Cheng, Yan Wen, Sicheng Quesenberry, Peter J. |
author_facet | Goldberg, Laura R. Dooner, Mark S. Papa, Elaine Pereira, Mandy Del Tatto, Michael Cheng, Yan Wen, Sicheng Quesenberry, Peter J. |
author_sort | Goldberg, Laura R. |
collection | PubMed |
description | Hematopoietic stem cells express differentiation markers B220 and Gr1 and are proliferative. We have shown that the expression of these entities changes with cell cycle passage. Overall, we conclude that primitive hematopoietic stem cells alter their differentiation potential with cell cycle progression. GRAPHICAL ABSTRACT: Murine derived long-term hematopoietic stem cells (LT-HSC) are cycling and thus always changing phenotype. Here we show that over one half of marrow LT-HSC are in the population expressing differentiation epitopes and that B220 and Gr-1 positive populations are replete with LT-HSC after a single FACS separation but if subjected to a second separation these cells no longer contain LT-HSC. However, with second separated cells there is a population appearing that is B220 negative and replete with cycling c-Kit, Sca-1 CD150 positive LT-HSC. There is a 3–4 h interval between the first and second B220 or GR-1 FACS separation during which the stem cells continue to cycle. Thus, the LT-HSC have lost B220 or GR-1 expression as the cells progress through cell cycle, although they have maintained the c-kit, Sca-1 and CD150 stem cells markers over this time interval. These data indicate that cycling stem cells express differentiation epitopes and alter their differentiation potential with cell cycle passage. [Image: see text] |
format | Online Article Text |
id | pubmed-9489557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-94895572022-09-22 Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage Goldberg, Laura R. Dooner, Mark S. Papa, Elaine Pereira, Mandy Del Tatto, Michael Cheng, Yan Wen, Sicheng Quesenberry, Peter J. Stem Cell Rev Rep Article Hematopoietic stem cells express differentiation markers B220 and Gr1 and are proliferative. We have shown that the expression of these entities changes with cell cycle passage. Overall, we conclude that primitive hematopoietic stem cells alter their differentiation potential with cell cycle progression. GRAPHICAL ABSTRACT: Murine derived long-term hematopoietic stem cells (LT-HSC) are cycling and thus always changing phenotype. Here we show that over one half of marrow LT-HSC are in the population expressing differentiation epitopes and that B220 and Gr-1 positive populations are replete with LT-HSC after a single FACS separation but if subjected to a second separation these cells no longer contain LT-HSC. However, with second separated cells there is a population appearing that is B220 negative and replete with cycling c-Kit, Sca-1 CD150 positive LT-HSC. There is a 3–4 h interval between the first and second B220 or GR-1 FACS separation during which the stem cells continue to cycle. Thus, the LT-HSC have lost B220 or GR-1 expression as the cells progress through cell cycle, although they have maintained the c-kit, Sca-1 and CD150 stem cells markers over this time interval. These data indicate that cycling stem cells express differentiation epitopes and alter their differentiation potential with cell cycle passage. [Image: see text] Springer US 2022-05-03 2022 /pmc/articles/PMC9489557/ /pubmed/35503199 http://dx.doi.org/10.1007/s12015-022-10374-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Goldberg, Laura R. Dooner, Mark S. Papa, Elaine Pereira, Mandy Del Tatto, Michael Cheng, Yan Wen, Sicheng Quesenberry, Peter J. Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title | Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title_full | Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title_fullStr | Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title_full_unstemmed | Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title_short | Differentiation Epitopes Define Hematopoietic Stem Cells and Change with Cell Cycle Passage |
title_sort | differentiation epitopes define hematopoietic stem cells and change with cell cycle passage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489557/ https://www.ncbi.nlm.nih.gov/pubmed/35503199 http://dx.doi.org/10.1007/s12015-022-10374-4 |
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