CRISPR-Cas9 screen in human embryonic stem cells to identify genes required for neural differentiation

Human embryonic stem cells (hESCs) continuously self-renew in culture and can be induced to differentiate into multiple cell types, including neural progenitor cells (NPCs). Here, we present a protocol to perform a CRISPR-Cas9 screen in hESCs to identify regulators that promote SOX1 expression durin...

Descripción completa

Detalles Bibliográficos
Autores principales: Qi, Shutao, Sivakumar, Sushama, Yu, Hongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490198/
https://www.ncbi.nlm.nih.gov/pubmed/36115024
http://dx.doi.org/10.1016/j.xpro.2022.101682
Descripción
Sumario:Human embryonic stem cells (hESCs) continuously self-renew in culture and can be induced to differentiate into multiple cell types, including neural progenitor cells (NPCs). Here, we present a protocol to perform a CRISPR-Cas9 screen in hESCs to identify regulators that promote SOX1 expression during NPC formation. This screening protocol can be adapted with other endpoint reporters for the identification of genes involved in the commitment of hESCs to other cell lineages. For complete details on the use and execution of this protocol, please refer to Sivakumar et al. (2022).

Ejemplares similares