CRISPR-Cas9 screen in human embryonic stem cells to identify genes required for neural differentiation

Human embryonic stem cells (hESCs) continuously self-renew in culture and can be induced to differentiate into multiple cell types, including neural progenitor cells (NPCs). Here, we present a protocol to perform a CRISPR-Cas9 screen in hESCs to identify regulators that promote SOX1 expression durin...

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Detalles Bibliográficos
Autores principales: Qi, Shutao, Sivakumar, Sushama, Yu, Hongtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490198/
https://www.ncbi.nlm.nih.gov/pubmed/36115024
http://dx.doi.org/10.1016/j.xpro.2022.101682

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