FACS-assisted CRISPR-Cas9 genome editing of human induced pluripotent stem cells

This manuscript proposes an efficient and reproducible protocol for the generation of genetically modified human induced pluripotent stem cells (hiPSCs) by genome editing using CRISPR-Cas9 technology. Here, we describe the experimental strategy for generating knockout (KO) and knockin (KI) clonal po...

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Detalles Bibliográficos
Autores principales: Caillaud, Amandine, Lévêque, Antoine, Thédrez, Aurélie, Girardeau, Aurore, Canac, Robin, Bray, Lise, Baudic, Manon, Barc, Julien, Gaborit, Nathalie, Lamirault, Guillaume, Gardie, Betty, Idriss, Salam, Rimbert, Antoine, Le May, Cédric, Cariou, Bertrand, Si-Tayeb, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490201/
https://www.ncbi.nlm.nih.gov/pubmed/36115027
http://dx.doi.org/10.1016/j.xpro.2022.101680

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