The N-terminal substrate specificity of the SurE peptide cyclase

SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in...

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Detalles Bibliográficos
Autores principales: Fazal, Asif, Wheeler, Jake, Webb, Michael E., Seipke, Ryan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491159/
https://www.ncbi.nlm.nih.gov/pubmed/36062889
http://dx.doi.org/10.1039/d2ob01061e
Descripción
Sumario:SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue.

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