The N-terminal substrate specificity of the SurE peptide cyclase

SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in...

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Detalles Bibliográficos
Autores principales: Fazal, Asif, Wheeler, Jake, Webb, Michael E., Seipke, Ryan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491159/
https://www.ncbi.nlm.nih.gov/pubmed/36062889
http://dx.doi.org/10.1039/d2ob01061e
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author Fazal, Asif
Wheeler, Jake
Webb, Michael E.
Seipke, Ryan F.
author_facet Fazal, Asif
Wheeler, Jake
Webb, Michael E.
Seipke, Ryan F.
author_sort Fazal, Asif
collection PubMed
description SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue.
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spelling pubmed-94911592022-10-31 The N-terminal substrate specificity of the SurE peptide cyclase Fazal, Asif Wheeler, Jake Webb, Michael E. Seipke, Ryan F. Org Biomol Chem Chemistry SurE is a standalone peptide cyclase essential for the production of surugamide antibiotics. Although SurE catalyses the cyclisation of varied nonribosomal peptides in vivo, its substrate specificity is poorly understood. To address this issue, an on-resin SurE cyclisation assay was developed and in combination with SNAC thioesters and kinetic measurements was used to define the chemical space of the N-terminal substrate residue. The Royal Society of Chemistry 2022-09-05 /pmc/articles/PMC9491159/ /pubmed/36062889 http://dx.doi.org/10.1039/d2ob01061e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Fazal, Asif
Wheeler, Jake
Webb, Michael E.
Seipke, Ryan F.
The N-terminal substrate specificity of the SurE peptide cyclase
title The N-terminal substrate specificity of the SurE peptide cyclase
title_full The N-terminal substrate specificity of the SurE peptide cyclase
title_fullStr The N-terminal substrate specificity of the SurE peptide cyclase
title_full_unstemmed The N-terminal substrate specificity of the SurE peptide cyclase
title_short The N-terminal substrate specificity of the SurE peptide cyclase
title_sort n-terminal substrate specificity of the sure peptide cyclase
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491159/
https://www.ncbi.nlm.nih.gov/pubmed/36062889
http://dx.doi.org/10.1039/d2ob01061e
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