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An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper
Characterization and genetic engineering of plant transporters involved in the pesticide uptake and translocation facilitate pesticide relocation to the tissue where the pests feed, thus improving the bioavailability of the agrichemicals. We aimed to identify thiamethoxam (THX) transporters in rice...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491460/ https://www.ncbi.nlm.nih.gov/pubmed/35678495 http://dx.doi.org/10.1111/pbi.13869 |
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author | Xiao, Yuyan Zhang, Hanlin Li, Zhiwei Huang, Tinghong Akihiro, Takashi Xu, Jian Xu, Hanhong Lin, Fei |
author_facet | Xiao, Yuyan Zhang, Hanlin Li, Zhiwei Huang, Tinghong Akihiro, Takashi Xu, Jian Xu, Hanhong Lin, Fei |
author_sort | Xiao, Yuyan |
collection | PubMed |
description | Characterization and genetic engineering of plant transporters involved in the pesticide uptake and translocation facilitate pesticide relocation to the tissue where the pests feed, thus improving the bioavailability of the agrichemicals. We aimed to identify thiamethoxam (THX) transporters in rice and modify their expression for better brown planthopper (BPH) control with less pesticide application. A yeast library expressing 1385 rice transporters was screened, leading to the identification of an amino acid transporter‐like (ATL) gene, namely OsATL15, which facilitates THX uptake in both yeast cells and rice seedlings. In contrast to a decrease in THX content in osatl15 knockout mutants, ectopic expression of OsATL15 under the control of the CaMV 35S promoter or a vascular‐bundle‐specific promoter gdcsPpro significantly increased THX accumulation in rice plants, thus further enhancing the THX efficacy against BPH. OsATL15 was localized in rice cell membrane and abundant in the root transverse sections, vascular bundles of leaf blade, and stem longitudinal sections, but not in hull and brown rice at filling stages. Our study shows that OsATL15 plays an essential role in THX uptake and its systemic distribution in rice. OsATL15 could be valuable in achieving precise pest control by biotechnology approaches. |
format | Online Article Text |
id | pubmed-9491460 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-94914602022-09-30 An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper Xiao, Yuyan Zhang, Hanlin Li, Zhiwei Huang, Tinghong Akihiro, Takashi Xu, Jian Xu, Hanhong Lin, Fei Plant Biotechnol J Research Articles Characterization and genetic engineering of plant transporters involved in the pesticide uptake and translocation facilitate pesticide relocation to the tissue where the pests feed, thus improving the bioavailability of the agrichemicals. We aimed to identify thiamethoxam (THX) transporters in rice and modify their expression for better brown planthopper (BPH) control with less pesticide application. A yeast library expressing 1385 rice transporters was screened, leading to the identification of an amino acid transporter‐like (ATL) gene, namely OsATL15, which facilitates THX uptake in both yeast cells and rice seedlings. In contrast to a decrease in THX content in osatl15 knockout mutants, ectopic expression of OsATL15 under the control of the CaMV 35S promoter or a vascular‐bundle‐specific promoter gdcsPpro significantly increased THX accumulation in rice plants, thus further enhancing the THX efficacy against BPH. OsATL15 was localized in rice cell membrane and abundant in the root transverse sections, vascular bundles of leaf blade, and stem longitudinal sections, but not in hull and brown rice at filling stages. Our study shows that OsATL15 plays an essential role in THX uptake and its systemic distribution in rice. OsATL15 could be valuable in achieving precise pest control by biotechnology approaches. John Wiley and Sons Inc. 2022-07-05 2022-10 /pmc/articles/PMC9491460/ /pubmed/35678495 http://dx.doi.org/10.1111/pbi.13869 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Xiao, Yuyan Zhang, Hanlin Li, Zhiwei Huang, Tinghong Akihiro, Takashi Xu, Jian Xu, Hanhong Lin, Fei An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title | An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title_full | An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title_fullStr | An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title_full_unstemmed | An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title_short | An amino acid transporter‐like protein (OsATL15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
title_sort | amino acid transporter‐like protein (osatl15) facilitates the systematic distribution of thiamethoxam in rice for controlling the brown planthopper |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491460/ https://www.ncbi.nlm.nih.gov/pubmed/35678495 http://dx.doi.org/10.1111/pbi.13869 |
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