Reverse mutants of the catalytic 19 kDa mutant protein (nanoKAZ/nanoLuc) from Oplophorus luciferase with coelenterazine as preferred substrate

Native Oplophorus luciferase (OpLase) and its catalytic 19 kDa protein (wild KAZ) show highest luminescence activity with coelenterazine (CTZ) among CTZ analogs. Mutated wild KAZ with 16 amino acid substitutions (nanoKAZ/nanoLuc) utilizes bis-coelenterazine (bis-CTZ) as the preferred substrate and e...

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Detalles Bibliográficos
Autores principales: Inouye, Satoshi, Sato, Jun-ichi, Sahara-Miura, Yuiko, Tomabechi, Yuri, Sumida, Yuto, Sekine, Shun-ichi, Shirouzu, Mikako, Hosoya, Takamitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491549/
https://www.ncbi.nlm.nih.gov/pubmed/36129943
http://dx.doi.org/10.1371/journal.pone.0272992

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