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Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria

Xenorhabdus and Photorhabdus can produce a variety of secondary metabolites with broad spectrum bioactivity against microorganisms. We investigated the antibacterial activity of Xenorhabdus and Photorhabdus against 15 antibiotic-resistant bacteria strains. Photorhabdus extracts had strong inhibitory...

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Autores principales: Muangpat, Paramaporn, Meesil, Wipanee, Ngoenkam, Jatuporn, Teethaisong, Yothin, Thummeepak, Rapee, Sitthisak, Sutthirat, Tandhavanant, Sarunporn, Chantratita, Narisara, Bode, Helge B., Vitta, Apichat, Thanwisai, Aunchalee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491552/
https://www.ncbi.nlm.nih.gov/pubmed/36129957
http://dx.doi.org/10.1371/journal.pone.0274956
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author Muangpat, Paramaporn
Meesil, Wipanee
Ngoenkam, Jatuporn
Teethaisong, Yothin
Thummeepak, Rapee
Sitthisak, Sutthirat
Tandhavanant, Sarunporn
Chantratita, Narisara
Bode, Helge B.
Vitta, Apichat
Thanwisai, Aunchalee
author_facet Muangpat, Paramaporn
Meesil, Wipanee
Ngoenkam, Jatuporn
Teethaisong, Yothin
Thummeepak, Rapee
Sitthisak, Sutthirat
Tandhavanant, Sarunporn
Chantratita, Narisara
Bode, Helge B.
Vitta, Apichat
Thanwisai, Aunchalee
author_sort Muangpat, Paramaporn
collection PubMed
description Xenorhabdus and Photorhabdus can produce a variety of secondary metabolites with broad spectrum bioactivity against microorganisms. We investigated the antibacterial activity of Xenorhabdus and Photorhabdus against 15 antibiotic-resistant bacteria strains. Photorhabdus extracts had strong inhibitory the growth of Methicillin-resistant Staphylococcus aureus (MRSA) by disk diffusion. The P. akhurstii s subsp. akhurstii (bNN168.5_TH) extract showed lower minimum inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The interaction between either P. akhurstii subsp. akhurstii (bNN141.3_TH) or P. akhurstii subsp. akhurstii (bNN168.5_TH) or P. hainanensis (bNN163.3_TH) extract in combination with oxacillin determined by checkerboard assay exhibited partially synergistic interaction with fractional inhibitory concentration index (FICI) of 0.53. Time-killing assay for P. akhurstii subsp. akhurstii (bNN168.5_TH) extract against S. aureus strain PB36 significantly decreased cell viability from 10(5) CFU/ml to 10(3) CFU/ml within 30 min (P < 0.001, t-test). Transmission electron microscopic investigation elucidated that the bNN168.5_TH extract caused treated S. aureus strain PB36 (MRSA) cell membrane damage. The biosynthetic gene clusters of the bNN168.5_TH contained non-ribosomal peptide synthetase cluster (NRPS), hybrid NRPS-type l polyketide synthase (PKS) and siderophore, which identified potentially interesting bioactive products: xenematide, luminmide, xenortide A-D, luminmycin A, putrebactin/avaroferrin and rhizomide A-C. This study demonstrates that bNN168.5_TH showed antibacterial activity by disrupting bacterial cytoplasmic membrane and the draft genome provided insights into the classes of bioactive products. This also provides a potential approach in developing a novel antibacterial agent.
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spelling pubmed-94915522022-09-22 Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria Muangpat, Paramaporn Meesil, Wipanee Ngoenkam, Jatuporn Teethaisong, Yothin Thummeepak, Rapee Sitthisak, Sutthirat Tandhavanant, Sarunporn Chantratita, Narisara Bode, Helge B. Vitta, Apichat Thanwisai, Aunchalee PLoS One Research Article Xenorhabdus and Photorhabdus can produce a variety of secondary metabolites with broad spectrum bioactivity against microorganisms. We investigated the antibacterial activity of Xenorhabdus and Photorhabdus against 15 antibiotic-resistant bacteria strains. Photorhabdus extracts had strong inhibitory the growth of Methicillin-resistant Staphylococcus aureus (MRSA) by disk diffusion. The P. akhurstii s subsp. akhurstii (bNN168.5_TH) extract showed lower minimum inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC). The interaction between either P. akhurstii subsp. akhurstii (bNN141.3_TH) or P. akhurstii subsp. akhurstii (bNN168.5_TH) or P. hainanensis (bNN163.3_TH) extract in combination with oxacillin determined by checkerboard assay exhibited partially synergistic interaction with fractional inhibitory concentration index (FICI) of 0.53. Time-killing assay for P. akhurstii subsp. akhurstii (bNN168.5_TH) extract against S. aureus strain PB36 significantly decreased cell viability from 10(5) CFU/ml to 10(3) CFU/ml within 30 min (P < 0.001, t-test). Transmission electron microscopic investigation elucidated that the bNN168.5_TH extract caused treated S. aureus strain PB36 (MRSA) cell membrane damage. The biosynthetic gene clusters of the bNN168.5_TH contained non-ribosomal peptide synthetase cluster (NRPS), hybrid NRPS-type l polyketide synthase (PKS) and siderophore, which identified potentially interesting bioactive products: xenematide, luminmide, xenortide A-D, luminmycin A, putrebactin/avaroferrin and rhizomide A-C. This study demonstrates that bNN168.5_TH showed antibacterial activity by disrupting bacterial cytoplasmic membrane and the draft genome provided insights into the classes of bioactive products. This also provides a potential approach in developing a novel antibacterial agent. Public Library of Science 2022-09-21 /pmc/articles/PMC9491552/ /pubmed/36129957 http://dx.doi.org/10.1371/journal.pone.0274956 Text en © 2022 Muangpat et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Muangpat, Paramaporn
Meesil, Wipanee
Ngoenkam, Jatuporn
Teethaisong, Yothin
Thummeepak, Rapee
Sitthisak, Sutthirat
Tandhavanant, Sarunporn
Chantratita, Narisara
Bode, Helge B.
Vitta, Apichat
Thanwisai, Aunchalee
Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title_full Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title_fullStr Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title_full_unstemmed Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title_short Genome analysis of secondary metabolite‑biosynthetic gene clusters of Photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
title_sort genome analysis of secondary metabolite‑biosynthetic gene clusters of photorhabdus akhurstii subsp. akhurstii and its antibacterial activity against antibiotic-resistant bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491552/
https://www.ncbi.nlm.nih.gov/pubmed/36129957
http://dx.doi.org/10.1371/journal.pone.0274956
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