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Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing

Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processe...

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Detalles Bibliográficos
Autores principales: Cho, Young Kwan, Kim, Hyunho, Bénard, Alan, Woo, Hyun-Kyung, Czubayko, Franziska, David, Paul, Hansen, Frederik J., Lee, Jong Ik, Park, Jay Hoon, Schneck, Emmanuel, Weber, Georg F., Shin, Ik-Soo, Lee, Hakho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491722/
https://www.ncbi.nlm.nih.gov/pubmed/36129990
http://dx.doi.org/10.1126/sciadv.abq4022
Descripción
Sumario:Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processes were carried out in isolated chambers and coupled through an electrode. The strategy allowed us to minimize cross-chemical reactions, design electrodes for high ECL signals, and integrate multiple sensors in a chip. As a proof of concept, we implemented an eight-plex ECLipse and applied it to detect host factors in human plasma. ECLipse achieved higher signal-to-noise ratio than conventional ECL assays and was >7000-fold more sensitive than enzyme-linked immunosorbent assay. In a pilot clinical study, we could detect septic conditions by measuring host factors [i.e., interleukin-3 (IL-3), IL-6, and procalcitonin (PCT)]. ECLipse assay further revealed distinct IL-3 and IL-6 patterns in patients with severe acute respiratory syndrome coronavirus 2 infection.