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Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing
Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processe...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491722/ https://www.ncbi.nlm.nih.gov/pubmed/36129990 http://dx.doi.org/10.1126/sciadv.abq4022 |
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author | Cho, Young Kwan Kim, Hyunho Bénard, Alan Woo, Hyun-Kyung Czubayko, Franziska David, Paul Hansen, Frederik J. Lee, Jong Ik Park, Jay Hoon Schneck, Emmanuel Weber, Georg F. Shin, Ik-Soo Lee, Hakho |
author_facet | Cho, Young Kwan Kim, Hyunho Bénard, Alan Woo, Hyun-Kyung Czubayko, Franziska David, Paul Hansen, Frederik J. Lee, Jong Ik Park, Jay Hoon Schneck, Emmanuel Weber, Georg F. Shin, Ik-Soo Lee, Hakho |
author_sort | Cho, Young Kwan |
collection | PubMed |
description | Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processes were carried out in isolated chambers and coupled through an electrode. The strategy allowed us to minimize cross-chemical reactions, design electrodes for high ECL signals, and integrate multiple sensors in a chip. As a proof of concept, we implemented an eight-plex ECLipse and applied it to detect host factors in human plasma. ECLipse achieved higher signal-to-noise ratio than conventional ECL assays and was >7000-fold more sensitive than enzyme-linked immunosorbent assay. In a pilot clinical study, we could detect septic conditions by measuring host factors [i.e., interleukin-3 (IL-3), IL-6, and procalcitonin (PCT)]. ECLipse assay further revealed distinct IL-3 and IL-6 patterns in patients with severe acute respiratory syndrome coronavirus 2 infection. |
format | Online Article Text |
id | pubmed-9491722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-94917222022-10-03 Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing Cho, Young Kwan Kim, Hyunho Bénard, Alan Woo, Hyun-Kyung Czubayko, Franziska David, Paul Hansen, Frederik J. Lee, Jong Ik Park, Jay Hoon Schneck, Emmanuel Weber, Georg F. Shin, Ik-Soo Lee, Hakho Sci Adv Physical and Materials Sciences Electrochemiluminescence (ECL) has an inherently low background and enables precise chemical reactions through electrical control. Here, we report an advanced ECL system, termed ECLipse (ECL in paired signal electrode). We physically separated ECL generation from target detection: These two processes were carried out in isolated chambers and coupled through an electrode. The strategy allowed us to minimize cross-chemical reactions, design electrodes for high ECL signals, and integrate multiple sensors in a chip. As a proof of concept, we implemented an eight-plex ECLipse and applied it to detect host factors in human plasma. ECLipse achieved higher signal-to-noise ratio than conventional ECL assays and was >7000-fold more sensitive than enzyme-linked immunosorbent assay. In a pilot clinical study, we could detect septic conditions by measuring host factors [i.e., interleukin-3 (IL-3), IL-6, and procalcitonin (PCT)]. ECLipse assay further revealed distinct IL-3 and IL-6 patterns in patients with severe acute respiratory syndrome coronavirus 2 infection. American Association for the Advancement of Science 2022-09-21 /pmc/articles/PMC9491722/ /pubmed/36129990 http://dx.doi.org/10.1126/sciadv.abq4022 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Physical and Materials Sciences Cho, Young Kwan Kim, Hyunho Bénard, Alan Woo, Hyun-Kyung Czubayko, Franziska David, Paul Hansen, Frederik J. Lee, Jong Ik Park, Jay Hoon Schneck, Emmanuel Weber, Georg F. Shin, Ik-Soo Lee, Hakho Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title | Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title_full | Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title_fullStr | Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title_full_unstemmed | Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title_short | Electrochemiluminescence in paired signal electrode (ECLipse) enables modular and scalable biosensing |
title_sort | electrochemiluminescence in paired signal electrode (eclipse) enables modular and scalable biosensing |
topic | Physical and Materials Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491722/ https://www.ncbi.nlm.nih.gov/pubmed/36129990 http://dx.doi.org/10.1126/sciadv.abq4022 |
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