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Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test

Glucose tolerance tests (GTT) are commonly performed in beef cattle to evaluate the glucose-insulin signaling pathway. Blood samples are obtained via a catheter and then transferred back to the laboratory for further analysis. A hand-held glucometer used chute-side can make performing GTT’s and quan...

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Autores principales: Nickles, Kirsten R., Relling, Alejandro E., Garcia-Guerra, Alvaro, Fluharty, Francis L., Parker, Anthony J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491837/
https://www.ncbi.nlm.nih.gov/pubmed/35853033
http://dx.doi.org/10.1371/journal.pone.0271673
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author Nickles, Kirsten R.
Relling, Alejandro E.
Garcia-Guerra, Alvaro
Fluharty, Francis L.
Parker, Anthony J.
author_facet Nickles, Kirsten R.
Relling, Alejandro E.
Garcia-Guerra, Alvaro
Fluharty, Francis L.
Parker, Anthony J.
author_sort Nickles, Kirsten R.
collection PubMed
description Glucose tolerance tests (GTT) are commonly performed in beef cattle to evaluate the glucose-insulin signaling pathway. Blood samples are obtained via a catheter and then transferred back to the laboratory for further analysis. A hand-held glucometer used chute-side can make performing GTT’s and quantifying blood glucose concentration much easier and faster for research purposes. The purpose of this study was to evaluate the agreement between a hand-held electronic glucometer (Precision Xtra; Abbott Diabetes Care Inc., Mississauga, ON, Canada) for chute-side use in beef cattle compared with a colorimetric assay in the laboratory (Stanbio Glucose LiquiColor; Stanbio Laboratory, Boerne, TX, USA). A GTT was performed on 13 Simmental × Angus steers during the growing phase. Blood samples were obtained via a jugular catheter. Glucometer readings were taken immediately after blood was sampled from the jugular with no preservative, and laboratory measurements were conducted on plasma preserved with sodium fluoride. A paired t-test (P = 0.40), Pearson’s correlation (P < 0.001; r = 0.95), Bland-Altman plot, and Lin’s concordance correlation coefficient (LCCC = 0.90) were completed to evaluate the performance of the glucometer relative to the results from the laboratory assay. Based on the results, we conclude that the glucometer is an acceptable method for measuring blood glucose concentration in beef cattle under field conditions.
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spelling pubmed-94918372022-09-22 Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test Nickles, Kirsten R. Relling, Alejandro E. Garcia-Guerra, Alvaro Fluharty, Francis L. Parker, Anthony J. PLoS One Research Article Glucose tolerance tests (GTT) are commonly performed in beef cattle to evaluate the glucose-insulin signaling pathway. Blood samples are obtained via a catheter and then transferred back to the laboratory for further analysis. A hand-held glucometer used chute-side can make performing GTT’s and quantifying blood glucose concentration much easier and faster for research purposes. The purpose of this study was to evaluate the agreement between a hand-held electronic glucometer (Precision Xtra; Abbott Diabetes Care Inc., Mississauga, ON, Canada) for chute-side use in beef cattle compared with a colorimetric assay in the laboratory (Stanbio Glucose LiquiColor; Stanbio Laboratory, Boerne, TX, USA). A GTT was performed on 13 Simmental × Angus steers during the growing phase. Blood samples were obtained via a jugular catheter. Glucometer readings were taken immediately after blood was sampled from the jugular with no preservative, and laboratory measurements were conducted on plasma preserved with sodium fluoride. A paired t-test (P = 0.40), Pearson’s correlation (P < 0.001; r = 0.95), Bland-Altman plot, and Lin’s concordance correlation coefficient (LCCC = 0.90) were completed to evaluate the performance of the glucometer relative to the results from the laboratory assay. Based on the results, we conclude that the glucometer is an acceptable method for measuring blood glucose concentration in beef cattle under field conditions. Public Library of Science 2022-07-19 /pmc/articles/PMC9491837/ /pubmed/35853033 http://dx.doi.org/10.1371/journal.pone.0271673 Text en © 2022 Nickles et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nickles, Kirsten R.
Relling, Alejandro E.
Garcia-Guerra, Alvaro
Fluharty, Francis L.
Parker, Anthony J.
Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title_full Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title_fullStr Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title_full_unstemmed Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title_short Short communication: A comparison between two glucose measurement methods in beef steers during a glucose tolerance test
title_sort short communication: a comparison between two glucose measurement methods in beef steers during a glucose tolerance test
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491837/
https://www.ncbi.nlm.nih.gov/pubmed/35853033
http://dx.doi.org/10.1371/journal.pone.0271673
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