Cargando…

Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics

OBJECTIVE: To explore the possible mechanism of fluid shear stress on human nucleus pulposus cells based on label-free proteomics technology. METHODS: The human nucleus pulposus cell line was purchased and subcultured in vitro. The Flexcell STR-4000 multiflow field cell fluid shear stress loading cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Xie, Liang-yu, Cao, Sheng-nan, Li, Zi-teng, Wang, Dan-dan, Shi, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492342/
https://www.ncbi.nlm.nih.gov/pubmed/36157212
http://dx.doi.org/10.1155/2022/3860898
_version_ 1784793457440587776
author Xie, Liang-yu
Cao, Sheng-nan
Li, Zi-teng
Wang, Dan-dan
Shi, Bin
author_facet Xie, Liang-yu
Cao, Sheng-nan
Li, Zi-teng
Wang, Dan-dan
Shi, Bin
author_sort Xie, Liang-yu
collection PubMed
description OBJECTIVE: To explore the possible mechanism of fluid shear stress on human nucleus pulposus cells based on label-free proteomics technology. METHODS: The human nucleus pulposus cell line was purchased and subcultured in vitro. The Flexcell STR-4000 multiflow field cell fluid shear stress loading culture system was used to apply continuous laminar fluid shear stress (12 dyne/cm(2), 45 mins) to the monolayer adherent cells. Those without mechanical loading were used as the control group, and those subjected to fluid shear loading were used as the experimental group. Differential protein expression was identified using mass spectrometry identification technology, and bioinformatics analysis was performed using Gene Ontology GO (Gene Ontology) and Kyoto Encyclopedia of Genes and Genomes KEGG (Kyoto Encyclopedia of Genes and Genomes). RESULTS: The proteomics results of the experimental group and the control group showed that the total number of mass spectra was 638653, the number of matched mass spectra was 170110, the total number of identified peptides was 32050, the specific peptide was 30564, and the total number of identified proteins was 4745. Comparing the two groups, 47 proteins were significantly differentially expressed, namely, 25 upregulated proteins and 22 downregulated proteins. Bioinformatics analysis showed that significantly different proteins were mainly manifested in cellular process, biological regulation, metabolic process, binding, catalytic activity, cellular components (cell part), organelle part (organelle part), and other molecular biological functions. CONCLUSION: Using proteomics technology to screen human nucleus pulposus cells after fluid shear stress loading, the differential protein expression provides a basis for further exploration of the mechanism of mechanical factors on nucleus pulposus.
format Online
Article
Text
id pubmed-9492342
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-94923422022-09-22 Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics Xie, Liang-yu Cao, Sheng-nan Li, Zi-teng Wang, Dan-dan Shi, Bin Dis Markers Research Article OBJECTIVE: To explore the possible mechanism of fluid shear stress on human nucleus pulposus cells based on label-free proteomics technology. METHODS: The human nucleus pulposus cell line was purchased and subcultured in vitro. The Flexcell STR-4000 multiflow field cell fluid shear stress loading culture system was used to apply continuous laminar fluid shear stress (12 dyne/cm(2), 45 mins) to the monolayer adherent cells. Those without mechanical loading were used as the control group, and those subjected to fluid shear loading were used as the experimental group. Differential protein expression was identified using mass spectrometry identification technology, and bioinformatics analysis was performed using Gene Ontology GO (Gene Ontology) and Kyoto Encyclopedia of Genes and Genomes KEGG (Kyoto Encyclopedia of Genes and Genomes). RESULTS: The proteomics results of the experimental group and the control group showed that the total number of mass spectra was 638653, the number of matched mass spectra was 170110, the total number of identified peptides was 32050, the specific peptide was 30564, and the total number of identified proteins was 4745. Comparing the two groups, 47 proteins were significantly differentially expressed, namely, 25 upregulated proteins and 22 downregulated proteins. Bioinformatics analysis showed that significantly different proteins were mainly manifested in cellular process, biological regulation, metabolic process, binding, catalytic activity, cellular components (cell part), organelle part (organelle part), and other molecular biological functions. CONCLUSION: Using proteomics technology to screen human nucleus pulposus cells after fluid shear stress loading, the differential protein expression provides a basis for further exploration of the mechanism of mechanical factors on nucleus pulposus. Hindawi 2022-09-14 /pmc/articles/PMC9492342/ /pubmed/36157212 http://dx.doi.org/10.1155/2022/3860898 Text en Copyright © 2022 Liang-yu Xie et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xie, Liang-yu
Cao, Sheng-nan
Li, Zi-teng
Wang, Dan-dan
Shi, Bin
Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title_full Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title_fullStr Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title_full_unstemmed Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title_short Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics
title_sort effects of fluid shear stress on human intervertebral disc nucleus pulposus cells based on label-free quantitative proteomics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492342/
https://www.ncbi.nlm.nih.gov/pubmed/36157212
http://dx.doi.org/10.1155/2022/3860898
work_keys_str_mv AT xieliangyu effectsoffluidshearstressonhumanintervertebraldiscnucleuspulposuscellsbasedonlabelfreequantitativeproteomics
AT caoshengnan effectsoffluidshearstressonhumanintervertebraldiscnucleuspulposuscellsbasedonlabelfreequantitativeproteomics
AT liziteng effectsoffluidshearstressonhumanintervertebraldiscnucleuspulposuscellsbasedonlabelfreequantitativeproteomics
AT wangdandan effectsoffluidshearstressonhumanintervertebraldiscnucleuspulposuscellsbasedonlabelfreequantitativeproteomics
AT shibin effectsoffluidshearstressonhumanintervertebraldiscnucleuspulposuscellsbasedonlabelfreequantitativeproteomics