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Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways

Rosa roxburghii Tratt fruit is a highly valued fruit that contains abundant functional and nutritional constituents. In this study, a novel polysaccharide, named RTFP-1, was isolated and purified from R. roxburghii Tratt fruit. Structural characterization indicated that RTFP-1 was a homogeneous hete...

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Autores principales: Jin, Yanlin, Li, Yinghua, Wang, Lei, Fu, Xiong, Li, Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492980/
https://www.ncbi.nlm.nih.gov/pubmed/36161228
http://dx.doi.org/10.1016/j.crfs.2022.09.016
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author Jin, Yanlin
Li, Yinghua
Wang, Lei
Fu, Xiong
Li, Chao
author_facet Jin, Yanlin
Li, Yinghua
Wang, Lei
Fu, Xiong
Li, Chao
author_sort Jin, Yanlin
collection PubMed
description Rosa roxburghii Tratt fruit is a highly valued fruit that contains abundant functional and nutritional constituents. In this study, a novel polysaccharide, named RTFP-1, was isolated and purified from R. roxburghii Tratt fruit. Structural characterization indicated that RTFP-1 was a homogeneous heteropolysaccharide with the molecular weight (Mw) of 128.7 kDa and consisted of arabinose, galactose, glucose, mannose, xylose, and fucose with molar ratio percentages of 34.84, 40.59, 12.11, 5.06, 3.39, and 4.01%, respectively. A CCK-8 assay indicated that RTFP-1 inhibited the cell growth of HepG2 cells in a dose-dependent manner. Morphological analysis and flow cytometry experiment showed that RTFP-1 promoted the apoptosis of HepG2 cells and increased reactive oxygen species (ROS) level. The underlying molecular mechanisms indicated that RTFP-1 activated the apoptosis of HepG2 cells through ROS-mediated MAPK, STAT, and p53 apoptotic pathways. These results suggest that RTFP-1 might be a potential chemopreventive and antitumor agent.
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spelling pubmed-94929802022-09-23 Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways Jin, Yanlin Li, Yinghua Wang, Lei Fu, Xiong Li, Chao Curr Res Food Sci Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Rosa roxburghii Tratt fruit is a highly valued fruit that contains abundant functional and nutritional constituents. In this study, a novel polysaccharide, named RTFP-1, was isolated and purified from R. roxburghii Tratt fruit. Structural characterization indicated that RTFP-1 was a homogeneous heteropolysaccharide with the molecular weight (Mw) of 128.7 kDa and consisted of arabinose, galactose, glucose, mannose, xylose, and fucose with molar ratio percentages of 34.84, 40.59, 12.11, 5.06, 3.39, and 4.01%, respectively. A CCK-8 assay indicated that RTFP-1 inhibited the cell growth of HepG2 cells in a dose-dependent manner. Morphological analysis and flow cytometry experiment showed that RTFP-1 promoted the apoptosis of HepG2 cells and increased reactive oxygen species (ROS) level. The underlying molecular mechanisms indicated that RTFP-1 activated the apoptosis of HepG2 cells through ROS-mediated MAPK, STAT, and p53 apoptotic pathways. These results suggest that RTFP-1 might be a potential chemopreventive and antitumor agent. Elsevier 2022-09-15 /pmc/articles/PMC9492980/ /pubmed/36161228 http://dx.doi.org/10.1016/j.crfs.2022.09.016 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
Jin, Yanlin
Li, Yinghua
Wang, Lei
Fu, Xiong
Li, Chao
Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title_full Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title_fullStr Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title_full_unstemmed Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title_short Physicochemical characterization of a polysaccharide from Rosa roxburghii Tratt fruit and its antitumor activity by activating ROS mediated pathways
title_sort physicochemical characterization of a polysaccharide from rosa roxburghii tratt fruit and its antitumor activity by activating ros mediated pathways
topic Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492980/
https://www.ncbi.nlm.nih.gov/pubmed/36161228
http://dx.doi.org/10.1016/j.crfs.2022.09.016
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