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The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis

BACKGROUND: Postoperative cognitive dysfunction is widely recognized as severe postoperative central nervous dysfunction and has a significant impact on the ’patient's physical and mental health. METHODS: Postoperative models of tibial fracture in aged rats were established, including the contr...

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Autores principales: Lin, Chuantao, Wang, Jing, Wang, Yuping, Chen, Chanjuan, Gao, Xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493054/
https://www.ncbi.nlm.nih.gov/pubmed/36137346
http://dx.doi.org/10.1016/j.clinsp.2022.100104
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author Lin, Chuantao
Wang, Jing
Wang, Yuping
Chen, Chanjuan
Gao, Xiang
author_facet Lin, Chuantao
Wang, Jing
Wang, Yuping
Chen, Chanjuan
Gao, Xiang
author_sort Lin, Chuantao
collection PubMed
description BACKGROUND: Postoperative cognitive dysfunction is widely recognized as severe postoperative central nervous dysfunction and has a significant impact on the ’patient's physical and mental health. METHODS: Postoperative models of tibial fracture in aged rats were established, including the control group, model group, CCL11 protein injection group, and saline injection group. Morris water maze test was used to detect the behavioral characteristics of rats. Enzyme-Linked Immunosorbent Assay was used or determine the content of CCL11 and CXCL10. Immunofluorescence staining was used to detect the distribution of CD14+CD163+macrophages in colon tissues and CD11b+CCR3+microglia cells in hippocampal tissues. Western blot analyzed NOX1 and STAT3 expression in hippocampus tissues. RESULTS: Water maze test results confirmed severe cognitive impairment in CCL11 rats. The content of CCL11 and CXCL10 in the CCL11 group was much higher than that of the model group. The distribution of macrophage and microglia cells in the CCL11 model group was greater than that in the model group and the saline group. The expression of NOX1 and STAT3 in the CCL11 group was higher compared with the model group. CONCLUSION: Abnormal macrophage function and excessive CCL11 secretion were observed in the rats with lower limb fractures after surgery. Postoperative central inflammation in rats with lower limb fracture induced postoperative cognitive dysfunction through the gut-brain axis molecular mechanism.
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spelling pubmed-94930542022-09-30 The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis Lin, Chuantao Wang, Jing Wang, Yuping Chen, Chanjuan Gao, Xiang Clinics (Sao Paulo) Original Articles BACKGROUND: Postoperative cognitive dysfunction is widely recognized as severe postoperative central nervous dysfunction and has a significant impact on the ’patient's physical and mental health. METHODS: Postoperative models of tibial fracture in aged rats were established, including the control group, model group, CCL11 protein injection group, and saline injection group. Morris water maze test was used to detect the behavioral characteristics of rats. Enzyme-Linked Immunosorbent Assay was used or determine the content of CCL11 and CXCL10. Immunofluorescence staining was used to detect the distribution of CD14+CD163+macrophages in colon tissues and CD11b+CCR3+microglia cells in hippocampal tissues. Western blot analyzed NOX1 and STAT3 expression in hippocampus tissues. RESULTS: Water maze test results confirmed severe cognitive impairment in CCL11 rats. The content of CCL11 and CXCL10 in the CCL11 group was much higher than that of the model group. The distribution of macrophage and microglia cells in the CCL11 model group was greater than that in the model group and the saline group. The expression of NOX1 and STAT3 in the CCL11 group was higher compared with the model group. CONCLUSION: Abnormal macrophage function and excessive CCL11 secretion were observed in the rats with lower limb fractures after surgery. Postoperative central inflammation in rats with lower limb fracture induced postoperative cognitive dysfunction through the gut-brain axis molecular mechanism. Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2022-09-19 /pmc/articles/PMC9493054/ /pubmed/36137346 http://dx.doi.org/10.1016/j.clinsp.2022.100104 Text en © 2022 HCFMUSP. Published by Elsevier España, S.L.U. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Articles
Lin, Chuantao
Wang, Jing
Wang, Yuping
Chen, Chanjuan
Gao, Xiang
The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title_full The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title_fullStr The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title_full_unstemmed The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title_short The postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
title_sort postoperative cognitive dysfunction induced by central inflammation with possible involvement of the gut-brain axis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493054/
https://www.ncbi.nlm.nih.gov/pubmed/36137346
http://dx.doi.org/10.1016/j.clinsp.2022.100104
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