Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q

Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes...

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Autores principales: Pradhan, Arpit Kumar, Shi, Qinfang, Tartler, Katharina Johanna, Rammes, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493138/
https://www.ncbi.nlm.nih.gov/pubmed/36125933
http://dx.doi.org/10.1016/j.xpro.2022.101687
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author Pradhan, Arpit Kumar
Shi, Qinfang
Tartler, Katharina Johanna
Rammes, Gerhard
author_facet Pradhan, Arpit Kumar
Shi, Qinfang
Tartler, Katharina Johanna
Rammes, Gerhard
author_sort Pradhan, Arpit Kumar
collection PubMed
description Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein.
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spelling pubmed-94931382022-09-23 Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q Pradhan, Arpit Kumar Shi, Qinfang Tartler, Katharina Johanna Rammes, Gerhard STAR Protoc Protocol Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein. Elsevier 2022-09-19 /pmc/articles/PMC9493138/ /pubmed/36125933 http://dx.doi.org/10.1016/j.xpro.2022.101687 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Pradhan, Arpit Kumar
Shi, Qinfang
Tartler, Katharina Johanna
Rammes, Gerhard
Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title_full Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title_fullStr Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title_full_unstemmed Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title_short Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q
title_sort quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo aβ treatment via colocalization analysis with c1q
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493138/
https://www.ncbi.nlm.nih.gov/pubmed/36125933
http://dx.doi.org/10.1016/j.xpro.2022.101687
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