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Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q

Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes...

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Detalles Bibliográficos
Autores principales:	Pradhan, Arpit Kumar, Shi, Qinfang, Tartler, Katharina Johanna, Rammes, Gerhard
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493138/ https://www.ncbi.nlm.nih.gov/pubmed/36125933 http://dx.doi.org/10.1016/j.xpro.2022.101687

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