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Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells

Piwi-interacting RNAs (piRNAs) are small non-coding RNAs (sncRNAs) of about 26–32 nucleotides in length and represent the largest class of sncRNA molecules expressed in animal cells. piRNAs have been shown to play a crucial role to safeguard the genome, maintaining genome complexity and integrity, a...

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Autores principales: Corsello, Tiziana, Kudlicki, Andrzej S, Liu, Tianshuang, Casola, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493205/
https://www.ncbi.nlm.nih.gov/pubmed/36158569
http://dx.doi.org/10.3389/fmolb.2022.931354
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author Corsello, Tiziana
Kudlicki, Andrzej S
Liu, Tianshuang
Casola, Antonella
author_facet Corsello, Tiziana
Kudlicki, Andrzej S
Liu, Tianshuang
Casola, Antonella
author_sort Corsello, Tiziana
collection PubMed
description Piwi-interacting RNAs (piRNAs) are small non-coding RNAs (sncRNAs) of about 26–32 nucleotides in length and represent the largest class of sncRNA molecules expressed in animal cells. piRNAs have been shown to play a crucial role to safeguard the genome, maintaining genome complexity and integrity, as they suppress the insertional mutations caused by transposable elements. However, there is growing evidence for the role of piRNAs in controlling gene expression in somatic cells as well. Little is known about changes in piRNA expression and possible function occurring in response to viral infections. In this study, we investigated the piRNA expression profile, using a human piRNA microarray, in human small airway epithelial (SAE) cells infected with respiratory syncytial virus (RSV), a leading cause of acute respiratory tract infections in children. We found a time-dependent increase in piRNAs differentially expressed in RSV-infected SAE cells. We validated the top piRNAs upregulated and downregulated at 24 h post-infection by RT-qPCR and identified potential targets. We then used Gene Ontology (GO) tool to predict the biological processes of the predicted targets of the most represented piRNAs in infected cells over the time course of RSV infection. We found that the most significant groups of targets of regulated piRNAs are related to cytoskeletal or Golgi organization and nucleic acid/nucleotide binding at 15 and 24 h p.i. To identify common patterns of time-dependent responses to infection, we clustered the significantly regulated expression profiles. Each of the clusters of temporal profiles have a distinct set of potential targets of the piRNAs in the cluster Understanding changes in piRNA expression in RSV-infected airway epithelial cells will increase our knowledge of the piRNA role in viral infection and might identify novel therapeutic targets for viral lung-mediated diseases.
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spelling pubmed-94932052022-09-23 Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells Corsello, Tiziana Kudlicki, Andrzej S Liu, Tianshuang Casola, Antonella Front Mol Biosci Molecular Biosciences Piwi-interacting RNAs (piRNAs) are small non-coding RNAs (sncRNAs) of about 26–32 nucleotides in length and represent the largest class of sncRNA molecules expressed in animal cells. piRNAs have been shown to play a crucial role to safeguard the genome, maintaining genome complexity and integrity, as they suppress the insertional mutations caused by transposable elements. However, there is growing evidence for the role of piRNAs in controlling gene expression in somatic cells as well. Little is known about changes in piRNA expression and possible function occurring in response to viral infections. In this study, we investigated the piRNA expression profile, using a human piRNA microarray, in human small airway epithelial (SAE) cells infected with respiratory syncytial virus (RSV), a leading cause of acute respiratory tract infections in children. We found a time-dependent increase in piRNAs differentially expressed in RSV-infected SAE cells. We validated the top piRNAs upregulated and downregulated at 24 h post-infection by RT-qPCR and identified potential targets. We then used Gene Ontology (GO) tool to predict the biological processes of the predicted targets of the most represented piRNAs in infected cells over the time course of RSV infection. We found that the most significant groups of targets of regulated piRNAs are related to cytoskeletal or Golgi organization and nucleic acid/nucleotide binding at 15 and 24 h p.i. To identify common patterns of time-dependent responses to infection, we clustered the significantly regulated expression profiles. Each of the clusters of temporal profiles have a distinct set of potential targets of the piRNAs in the cluster Understanding changes in piRNA expression in RSV-infected airway epithelial cells will increase our knowledge of the piRNA role in viral infection and might identify novel therapeutic targets for viral lung-mediated diseases. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493205/ /pubmed/36158569 http://dx.doi.org/10.3389/fmolb.2022.931354 Text en Copyright © 2022 Corsello, Kudlicki, Liu and Casola. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Corsello, Tiziana
Kudlicki, Andrzej S
Liu, Tianshuang
Casola, Antonella
Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title_full Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title_fullStr Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title_full_unstemmed Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title_short Respiratory syncytial virus infection changes the piwi-interacting RNA content of airway epithelial cells
title_sort respiratory syncytial virus infection changes the piwi-interacting rna content of airway epithelial cells
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493205/
https://www.ncbi.nlm.nih.gov/pubmed/36158569
http://dx.doi.org/10.3389/fmolb.2022.931354
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