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Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus

Parasitic diseases have a major impact on human and animal health worldwide. Despite the availability of effective anti-parasitic drugs, their excessive and uncontrolled use has promoted the emergence of drug resistance, severely affecting ecosystems and human health. Thus, developing environmentall...

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Autores principales: Ortiz Caltempa, Anabel, Hernández, Marisela, Pérez, Ana Lilia, Aguilar, Liliana, Guzmán, Cynthia, Ayón-Núñez, Dolores Adriana, Fragoso, Gladis, Bobes, Raúl J., López, Maria Eugenia, Sciutto, Edda, Villareal, María Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493254/
https://www.ncbi.nlm.nih.gov/pubmed/36159651
http://dx.doi.org/10.3389/fcimb.2022.958741
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author Ortiz Caltempa, Anabel
Hernández, Marisela
Pérez, Ana Lilia
Aguilar, Liliana
Guzmán, Cynthia
Ayón-Núñez, Dolores Adriana
Fragoso, Gladis
Bobes, Raúl J.
López, Maria Eugenia
Sciutto, Edda
Villareal, María Luisa
author_facet Ortiz Caltempa, Anabel
Hernández, Marisela
Pérez, Ana Lilia
Aguilar, Liliana
Guzmán, Cynthia
Ayón-Núñez, Dolores Adriana
Fragoso, Gladis
Bobes, Raúl J.
López, Maria Eugenia
Sciutto, Edda
Villareal, María Luisa
author_sort Ortiz Caltempa, Anabel
collection PubMed
description Parasitic diseases have a major impact on human and animal health worldwide. Despite the availability of effective anti-parasitic drugs, their excessive and uncontrolled use has promoted the emergence of drug resistance, severely affecting ecosystems and human health. Thus, developing environmentally friendly antiparasitic treatments is urgently needed. Carica papaya has shown promising effects against infectious diseases. C. papaya embryogenic calluses were genetically modified by our research team to insert immunogenic peptides with the goal of developing an oral anti-cysticercosis vaccine. Among these callus cell lines, one labeled as CF-23, which expresses the KETc7 immunogenic peptide, induced the highest protection levels against experimental cysticercosis. In the process of designing a natural antiparasitic product based on C. papaya that simultaneously induced immunity against cysticercosis, both transformed (SF-23) and untransformed (SF-WT) suspension cultures were produced and optimized. Our results showed a better duplication time (td) for SF-23 (6.9 days) than SF-WT (13.02 days); thus, the SF-23 line was selected for scale-up in a 2-L airlift bioreactor, reaching a td of 4.4 days. This is the first time that a transgenic line of C. papaya has been grown in an airlift bioreactor, highlighting its potential for scale-up cultivation in this type of reactor. Considering the previously reported nematocidal activity of C. papaya tissues, their activity against the nematode Haemonchus contortus of aqueous extracts of SF-WT and SF-23 was explored in this study, with promising results. The information herein reported will allow us to continue the cultivation of the transgenic cell suspension line of C. papaya under reproducible conditions, to develop a new anti-parasitic product.
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spelling pubmed-94932542022-09-23 Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus Ortiz Caltempa, Anabel Hernández, Marisela Pérez, Ana Lilia Aguilar, Liliana Guzmán, Cynthia Ayón-Núñez, Dolores Adriana Fragoso, Gladis Bobes, Raúl J. López, Maria Eugenia Sciutto, Edda Villareal, María Luisa Front Cell Infect Microbiol Cellular and Infection Microbiology Parasitic diseases have a major impact on human and animal health worldwide. Despite the availability of effective anti-parasitic drugs, their excessive and uncontrolled use has promoted the emergence of drug resistance, severely affecting ecosystems and human health. Thus, developing environmentally friendly antiparasitic treatments is urgently needed. Carica papaya has shown promising effects against infectious diseases. C. papaya embryogenic calluses were genetically modified by our research team to insert immunogenic peptides with the goal of developing an oral anti-cysticercosis vaccine. Among these callus cell lines, one labeled as CF-23, which expresses the KETc7 immunogenic peptide, induced the highest protection levels against experimental cysticercosis. In the process of designing a natural antiparasitic product based on C. papaya that simultaneously induced immunity against cysticercosis, both transformed (SF-23) and untransformed (SF-WT) suspension cultures were produced and optimized. Our results showed a better duplication time (td) for SF-23 (6.9 days) than SF-WT (13.02 days); thus, the SF-23 line was selected for scale-up in a 2-L airlift bioreactor, reaching a td of 4.4 days. This is the first time that a transgenic line of C. papaya has been grown in an airlift bioreactor, highlighting its potential for scale-up cultivation in this type of reactor. Considering the previously reported nematocidal activity of C. papaya tissues, their activity against the nematode Haemonchus contortus of aqueous extracts of SF-WT and SF-23 was explored in this study, with promising results. The information herein reported will allow us to continue the cultivation of the transgenic cell suspension line of C. papaya under reproducible conditions, to develop a new anti-parasitic product. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493254/ /pubmed/36159651 http://dx.doi.org/10.3389/fcimb.2022.958741 Text en Copyright © 2022 Ortiz Caltempa, Hernández, Pérez, Aguilar, Guzmán, Ayón-Núñez, Fragoso, Bobes, López, Sciutto and Villareal https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Ortiz Caltempa, Anabel
Hernández, Marisela
Pérez, Ana Lilia
Aguilar, Liliana
Guzmán, Cynthia
Ayón-Núñez, Dolores Adriana
Fragoso, Gladis
Bobes, Raúl J.
López, Maria Eugenia
Sciutto, Edda
Villareal, María Luisa
Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title_full Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title_fullStr Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title_full_unstemmed Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title_short Improvement of cell suspension cultures of transformed and untransformed Carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode Haemonchus contortus
title_sort improvement of cell suspension cultures of transformed and untransformed carica papaya cell lines, towards the development of an antiparasitic product against the gastrointestinal nematode haemonchus contortus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493254/
https://www.ncbi.nlm.nih.gov/pubmed/36159651
http://dx.doi.org/10.3389/fcimb.2022.958741
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