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Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics

The development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts,...

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Autores principales: Cosentino, Matheus Augusto Calvano, D’arc, Mirela, Moreira, Filipe Romero Rebello, Cavalcante, Liliane Tavares de Faria, Mouta, Ricardo, Coimbra, Amanda, Schiffler, Francine Bittencourt, Miranda, Thamiris dos Santos, Medeiros, Gabriel, Dias, Cecilia A., Souza, Antonizete R., Tavares, Maria Clotilde Henriques, Tanuri, Amilcar, Soares, Marcelo Alves, dos Santos, André Felipe Andrade
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493276/
https://www.ncbi.nlm.nih.gov/pubmed/36160188
http://dx.doi.org/10.3389/fmicb.2022.1002963
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author Cosentino, Matheus Augusto Calvano
D’arc, Mirela
Moreira, Filipe Romero Rebello
Cavalcante, Liliane Tavares de Faria
Mouta, Ricardo
Coimbra, Amanda
Schiffler, Francine Bittencourt
Miranda, Thamiris dos Santos
Medeiros, Gabriel
Dias, Cecilia A.
Souza, Antonizete R.
Tavares, Maria Clotilde Henriques
Tanuri, Amilcar
Soares, Marcelo Alves
dos Santos, André Felipe Andrade
author_facet Cosentino, Matheus Augusto Calvano
D’arc, Mirela
Moreira, Filipe Romero Rebello
Cavalcante, Liliane Tavares de Faria
Mouta, Ricardo
Coimbra, Amanda
Schiffler, Francine Bittencourt
Miranda, Thamiris dos Santos
Medeiros, Gabriel
Dias, Cecilia A.
Souza, Antonizete R.
Tavares, Maria Clotilde Henriques
Tanuri, Amilcar
Soares, Marcelo Alves
dos Santos, André Felipe Andrade
author_sort Cosentino, Matheus Augusto Calvano
collection PubMed
description The development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts, due to their evolutionary proximity to Old World primates, including humans. In the present work, novel anelloviruses were detected and characterized through HTS protocols in the NP Callithrix penicillata, the common black-tufted marmoset. De novo assembly of generated sequences was carried out, and a total of 15 contigs were identified with complete Anelloviridae ORF1 gene, two of them including a flanking GC-rich region, confirming the presence of two whole novel genomes of ~3 kb. The identified viruses were monophyletic within the Epsilontorquevirus genus, a lineage harboring previously reported anelloviruses infecting hosts from the Cebidae family. The genetic divergence found in the new viruses characterized two novel species, named Epsilontorquevirus callithrichensis I and II. The phylogenetic pattern inferred for the Epsilontorquevirus genus was consistent with the topology of their host species tree, echoing a virus-host diversification model observed in other viral groups. This study expands the host span of Anelloviridae and provides insights into their diversification dynamics, highlighting the importance of sampling animal viral genomes to obtain a clearer depiction of their long-term evolutionary processes.
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spelling pubmed-94932762022-09-23 Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics Cosentino, Matheus Augusto Calvano D’arc, Mirela Moreira, Filipe Romero Rebello Cavalcante, Liliane Tavares de Faria Mouta, Ricardo Coimbra, Amanda Schiffler, Francine Bittencourt Miranda, Thamiris dos Santos Medeiros, Gabriel Dias, Cecilia A. Souza, Antonizete R. Tavares, Maria Clotilde Henriques Tanuri, Amilcar Soares, Marcelo Alves dos Santos, André Felipe Andrade Front Microbiol Microbiology The development of high-throughput sequencing (HTS) technologies and metagenomics protocols deeply impacted the discovery of viral diversity. Moreover, the characterization of novel viruses in the Neotropical primates (NP) is central for the comprehension of viral evolution dynamics in those hosts, due to their evolutionary proximity to Old World primates, including humans. In the present work, novel anelloviruses were detected and characterized through HTS protocols in the NP Callithrix penicillata, the common black-tufted marmoset. De novo assembly of generated sequences was carried out, and a total of 15 contigs were identified with complete Anelloviridae ORF1 gene, two of them including a flanking GC-rich region, confirming the presence of two whole novel genomes of ~3 kb. The identified viruses were monophyletic within the Epsilontorquevirus genus, a lineage harboring previously reported anelloviruses infecting hosts from the Cebidae family. The genetic divergence found in the new viruses characterized two novel species, named Epsilontorquevirus callithrichensis I and II. The phylogenetic pattern inferred for the Epsilontorquevirus genus was consistent with the topology of their host species tree, echoing a virus-host diversification model observed in other viral groups. This study expands the host span of Anelloviridae and provides insights into their diversification dynamics, highlighting the importance of sampling animal viral genomes to obtain a clearer depiction of their long-term evolutionary processes. Frontiers Media S.A. 2022-09-08 /pmc/articles/PMC9493276/ /pubmed/36160188 http://dx.doi.org/10.3389/fmicb.2022.1002963 Text en Copyright © 2022 Cosentino, D’arc, Moreira, Cavalcante, Mouta, Coimbra, Schiffler, Miranda, Medeiros, Dias, Souza, Tavares, Tanuri, Soares and Santos. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Cosentino, Matheus Augusto Calvano
D’arc, Mirela
Moreira, Filipe Romero Rebello
Cavalcante, Liliane Tavares de Faria
Mouta, Ricardo
Coimbra, Amanda
Schiffler, Francine Bittencourt
Miranda, Thamiris dos Santos
Medeiros, Gabriel
Dias, Cecilia A.
Souza, Antonizete R.
Tavares, Maria Clotilde Henriques
Tanuri, Amilcar
Soares, Marcelo Alves
dos Santos, André Felipe Andrade
Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title_full Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title_fullStr Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title_full_unstemmed Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title_short Discovery of two novel Torque Teno viruses in Callithrix penicillata provides insights on Anelloviridae diversification dynamics
title_sort discovery of two novel torque teno viruses in callithrix penicillata provides insights on anelloviridae diversification dynamics
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9493276/
https://www.ncbi.nlm.nih.gov/pubmed/36160188
http://dx.doi.org/10.3389/fmicb.2022.1002963
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